Abstract | BACKGROUND/AIM: MATERIAL AND METHODS:
Paraffin sections of uterine cervical cancer and 130 were analyzed. Immunohistochemical staining protocols were evaluated with tissue slides from patients with cervical dysplasia (CIN III) and squamous epithelial carcinoma tissue with HPV infection. Liver and placental tissues were used as negative controls. E6-Specific antibody (Biorbyt) was used as primary antibody. The polymer staining method and diaminobenzidine were applied for further development. Panels of E7-specific antibodies were tested. Again, the polymer staining method and diaminobenzidine were applied for further development. RESULTS: E6-Specific antibody revealed specific and intense staining after pre-incubation of tissue slides with citrate buffer solution. Only the E7 antibody obtained from Chemicon showed intense and specific staining in patients with CIN III and squamous epithelial carcinoma tissue. Pre-incubation with proteinase K diminished non-specific reaction. CONCLUSION: Our results revealed a useful staining protocol for the immunohistochemical evaluation of E6/E7 oncoprotein expression in uterine cervical cancer, as well as in HPV-positive oropharyngeal cancer. Advantages of this method compared to mRNA in situ hybridization of E6/E7 are the much lower costs, as well as the broader applicability in pathological practice.
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Authors | Annika Stiasny, Christina Kuhn, Doris Mayr, Christoph Alexiou, Christina Janko, Irmi Wiest, Udo Jeschke, Bernd Kost |
Journal | Anticancer research
(Anticancer Res)
Vol. 36
Issue 6
Pg. 3195-8
(Jun 2016)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 27272848
(Publication Type: Journal Article)
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Copyright | Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Chemical References |
- DNA-Binding Proteins
- E6 protein, Human papillomavirus type 18
- Oncogene Proteins, Viral
- Papillomavirus E7 Proteins
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Topics |
- DNA-Binding Proteins
(analysis)
- Female
- Humans
- Immunohistochemistry
- Oncogene Proteins, Viral
(analysis)
- Papillomavirus E7 Proteins
(analysis)
- Uterine Cervical Neoplasms
(virology)
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