Abstract | OBJECTIVES: METHODS: Rats were pretreated with concurrent doses of benidipine and edaravone (1 μg/kg/day + 1 mg/kg/day and 3 μg/kg/day + 3 mg/kg/day) by intravenous (i.v.) and intraperitoneal (i.p.) routes respectively for 28 days, followed by MI induction using ISO (85 mg/kg) by subcutaneous route for two days at 24 h intervals. After the treatment period, blood was withdrawn and the heart was preserved for biochemical estimations. RESULTS: The activities of the cardiac biomarkers ( lactate dehydrogenase and creatine kinase-MB), and the level of malondialdehyde (MDA) significantly increased, while antioxidant markers ( reduced glutathione, catalase, superoxidase dismutase, glutathione peroxidase, glutathione reductase) were significantly decreased in the ISO intoxicated group compared with the control group. Moreover, the level of C-reactive protein (CRP) and Caspase-3 activity significantly increased in ISO-intoxicated group. An ultrastructure study was also carried out. Pretreatment with a combination of benidipine and edaravone significantly attenuated the activities of the cardiac biomarkers and the level of MDA, and significantly increased the antioxidant markers compared with the ISO-intoxicated group. Furthermore, pretreatment with the combination of benidipine and edaravone significantly decreased the level of CRP and Caspase-3 activity as compared to the ISO-treated group. The ultrastructure study of myocardium revealed that pretreated groups preserved the mitochondrial shape, the membrane and its internal structures. CONCLUSION: Taken together these results suggest that the combination of benidipine and edaravone showed significant protective effect in ISO-induced MI.
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Authors | Md Quamrul Hassan, Md Sayeed Akhtar, Mohd Akhtar, Javed Ali, Syed Ehtaishamul Haque, Abul Kalam Najmi |
Journal | Therapeutic advances in cardiovascular disease
(Ther Adv Cardiovasc Dis)
Vol. 10
Issue 4
Pg. 214-23
(Aug 2016)
ISSN: 1753-9455 [Electronic] England |
PMID | 26868288
(Publication Type: Journal Article)
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Copyright | © The Author(s), 2016. |
Chemical References |
- Calcium Channel Blockers
- Dihydropyridines
- Free Radical Scavengers
- benidipine
- Malondialdehyde
- C-Reactive Protein
- Caspase 3
- Isoproterenol
- Edaravone
- Antipyrine
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Topics |
- Animals
- Antipyrine
(analogs & derivatives, pharmacology)
- Apoptosis
(drug effects)
- C-Reactive Protein
(analysis)
- Calcium Channel Blockers
(pharmacology)
- Caspase 3
(metabolism)
- Dihydropyridines
(pharmacology)
- Edaravone
- Female
- Free Radical Scavengers
(pharmacology)
- Isoproterenol
(pharmacology)
- Male
- Malondialdehyde
(analysis)
- Myocardial Infarction
(chemically induced, drug therapy, metabolism, pathology)
- Myocardium
(enzymology, ultrastructure)
- Oxidative Stress
(drug effects)
- Rats
- Rats, Wistar
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