Effect of SIRT1 Gene on Epithelial-Mesenchymal Transition of Human Prostate Cancer PC-3 Cells.

Abstract

BACKGROUND The epithelial-mesenchymal transition (EMT) has been shown to be involved in the process of invasion and metastasis of prostate cancer. SIRT1 is the mammalian homologue of the silent information regulator 2 (Sir2) gene, and is abnormally expressed in prostate cancer cells. Therefore, it is hypothesized that SIRT1 mediates the invasion/metastatic ability of prostate cancer via EMT regulation. This study thus investigated the effect of SIRT1 gene on the invasion and migration of prostate cancer cell line PC-3 via the small interference RNA (siRNA) against SIRT1. MATERIAL AND METHODS SiRNA construct was transfected into PC-3 cells, which were tested for the cell migration and invasion ability by scratch assay and Transwell migration assay, respectively. Expression levels of vimentin, E-cadherin, and N-cadherin were further quantified by Western blotting and RT-PCR. RESULTS Both mRNA and protein levels of SIRT1 were depressed after siRNA transfection, along with weakened migration and invasion ability of PC-3 cells. Elevated E-cadherin and suppressed N-cadherin and vimentin were observed in those transfected cells. CONCLUSIONS The silencing of SIRT1 gene in PC-3 cells can suppress the movement, migration, and invasion functions of prostate cancer cells, possibly via the down-regulation of mesenchymal markers vimentin and N-cadherin accompanied with up-regulation of epithelial marker N-cadherin, thus reversing the EMT process.

• Authors: Ying Cui, Jiang Li, Fei Zheng, Yongri Ouyang, Xi Chen, Lei Zhang, Yang Chen, Lin Wang, Shijie Mu, Huizhong Zhang
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 22 Pg. 380-6 (Feb 05 2016) ISSN: 1643-3750 [Electronic] United States
• PMID: 26847404 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cadherins
• RNA, Small Interfering
• Vimentin
• Cellulose 1,4-beta-Cellobiosidase
• SIRT1 protein, human
• Sirtuin 1

Topics

• Cadherins (metabolism)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cellulose 1,4-beta-Cellobiosidase (metabolism)
• Down-Regulation
• Epithelial-Mesenchymal Transition (genetics)
• Humans
• Male
• Prostatic Neoplasms (genetics, metabolism, pathology)
• RNA, Small Interfering
• Sirtuin 1 (genetics, metabolism)
• Transfection
• Up-Regulation
• Vimentin (metabolism)