Abstract | BACKGROUND: METHODS: Six-week-old Sprague-Dawley rats were rendered CKD by subtotal nephrectomy (Nx) and compared with sham-operated rats. Cultured 3T3-L1 fibroblasts were differentiated into mature adipocytes with or without ADMA. Transgenic (Tg) mice overexpressing each isoform of ADMA degrading enzyme, dimethylarginine dimethylaminohydrolase 1 (DDAH1) and DDAH2 were subject to Nx and their phenotypes were investigated. RESULTS: In Nx rats, IR was evident and insulin stimulation failed to activate insulin signaling in adipose tissues. Adipose tissue weight, adipocyte size and adipocyte differentiation marker expressions decreased as a consequence of IR in Nx. Tissue lipid content in the liver and muscle increased in Nx rats. Tissue levels of ADMA, IS and oxidative stress increased in the adipose tissue of Nx rats. Both DDAH1 and DDAH2 expressions decreased, and a putative IS receptor, aryl hydrocarbon receptor, expression increased in the adipose tissue of Nx rats. ADMA inhibited adipocyte differentiation, triglyceride accumulation and insulin signaling, which were reversed by pretreatment with cGMP. In each type of Tg mice overexpressing DDAH1 or DDAH2, all lipodystrophy-like phenotypes induced by Nx were reversed. CONCLUSIONS: In mild CKD, dysregulation of the ADMA/DDAH pathway in adipose tissue triggers lipodystrophy-like phenotype changes, including ectopic fat depositions.
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Authors | Hitoshi Minakuchi, Shu Wakino, Kozi Hosoya, Keiko Sueyasu, Kazuhiro Hasegawa, Keisuke Shinozuka, Ayumi Yoshifuji, Koji Futatsugi, Motoaki Komatsu, Takeshi Kanda, Hirobumi Tokuyama, Koichi Hayashi, Hiroshi Itoh |
Journal | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
(Nephrol Dial Transplant)
Vol. 31
Issue 3
Pg. 413-23
(Mar 2016)
ISSN: 1460-2385 [Electronic] England |
PMID | 26516203
(Publication Type: Journal Article)
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Copyright | © The Author 2015. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. |
Chemical References |
- RNA
- N,N-dimethylarginine
- Arginine
- Amidohydrolases
- dimethylargininase
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Topics |
- Adipose Tissue
(metabolism)
- Amidohydrolases
(biosynthesis, genetics)
- Animals
- Arginine
(analogs & derivatives, biosynthesis, genetics)
- Blotting, Western
- Cells, Cultured
- Gene Expression Regulation
- Male
- Mice
- Mice, Transgenic
- Nephrectomy
(adverse effects)
- Oxidative Stress
(genetics)
- Phenotype
- RNA
(genetics)
- Rats
- Rats, Sprague-Dawley
- Real-Time Polymerase Chain Reaction
- Renal Insufficiency, Chronic
(genetics, metabolism)
- Signal Transduction
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