Abstract | BACKGROUND: METHODS: MsrA and the fusion protein PEP-1-MsrA were expressed and purified using a pET28a expression system. Transduction of the fusion protein into macrophages was confirmed by Western blot and immunofluorescence staining. Intracellular reactive oxygen species (ROS) and apoptosis levels were measured by flow cytometry. In in vivo study, MsrA or PEP-1-MsrA proteins were intraperitoneally injected into apoE(-/-) mice fed a Western diet for 12 weeks. Plasma lipids levels, inflammatory gene expression, and paraoxonase-1 (PON1) and superoxide dismutase (SOD) activities were assessed. Atherosclerotic lesions were analyzed by Oil Red O staining and immunohistochemistry. RESULTS: PEP-1-MsrA could penetrate the cells and significantly reduced intracellular ROS levels and apoptosis in H2O2-treated macrophages. It also decreased TNFα and IL-1β mRNA levels and increased the IL-10 mRNA level in lipopolysaccharide-treated macrophages. In in vivo study, PEP-1-MsrA injection significantly increased plasma PON1 and SOD activities and decreased plasma monocyte chemoattractant protein 1 (MCP-1) level compared to the injection of vehicle control or MsrA. In PEP-1-MsrA injected mice, hepatic PON1 levels were increased, while the expression of TNFα and IL-6 mRNA in the liver was suppressed. Although plasma total cholesterol and triglyceride levels did not change, the aortic atherosclerosis in PEP-1-MsrA treated mice was significantly reduced. This was accompanied by a reduction of total and apoptotic macrophages in the lesions. CONCLUSION:
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Authors | Yao Wu, Guanghui Xie, Yanyong Xu, Li Ma, Chuanfeng Tong, Daping Fan, Fen Du, Hong Yu |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 13
Pg. 316
(Sep 26 2015)
ISSN: 1479-5876 [Electronic] England |
PMID | 26410585
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoproteins E
- Pep-1 peptide
- Peptides
- Reactive Oxygen Species
- Recombinant Fusion Proteins
- Cysteamine
- Superoxide Dismutase
- Methionine Sulfoxide Reductases
- methionine sulfoxide reductase
- Aryldialkylphosphatase
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Topics |
- Animals
- Apolipoproteins E
(genetics)
- Apoptosis
- Aryldialkylphosphatase
(metabolism)
- Atherosclerosis
(genetics, prevention & control)
- Cell Line
- Cysteamine
(analogs & derivatives, metabolism)
- Disease Models, Animal
- Escherichia coli
(metabolism)
- HeLa Cells
- Humans
- Inflammation
(drug therapy)
- Liver
(metabolism)
- Macrophages
(metabolism)
- Male
- Methionine Sulfoxide Reductases
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Oxidation-Reduction
- Oxidative Stress
- Peptides
(metabolism)
- Protein Structure, Tertiary
- Reactive Oxygen Species
(metabolism)
- Recombinant Fusion Proteins
(metabolism)
- Superoxide Dismutase
(metabolism)
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