Abstract |
Low frequency electromagnetic field (LF-EMF) decreases the formation of reactive oxygen species, which are key mediators of ischemia/reperfusion (I/R) injury. Therefore, we hypothesized that the LF-EMF protects contractility of hearts subjected to I/R injury. Isolated rat hearts were subjected to 20 min of global no-flow ischemia, followed by 30 min reperfusion, in the presence or absence of LF-EMF. Coronary flow, heart rate, left ventricular developed pressure (LVDP), and rate pressure product (RPP) were determined for evaluation of heart mechanical function. The activity of cardiac matrix metalloproteinase-2 (MMP-2) and the contents of coronary effluent troponin I (TnI) and interleukin-6 (IL-6) were measured as markers of heart injury. LF-EMF prevented decreased RPP in I/R hearts, while having no effect on coronary flow. In addition, hearts subjected to I/R exhibited significantly increased LVDP when subjected to LF-EMF. Although TnI and IL-6 levels were increased in I/R hearts, their levels returned to baseline aerobic levels in I/R hearts subjected to LF-EMF. The reduced activity of MMP-2 in I/R hearts was reversed in hearts subjected to LF-EMF. The data presented here indicate that acute exposure to LF-EMF protects mechanical function of I/R hearts and reduces I/R injury.
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Authors | Dariusz Bialy, Magdalena Wawrzynska, Iwona Bil-Lula, Anna Krzywonos-Zawadzka, Mieczyslaw Wozniak, Virgilio J J Cadete, Grzegorz Sawicki |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2015
Pg. 396593
( 2015)
ISSN: 2314-6141 [Electronic] United States |
PMID | 25961016
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-6
- Reactive Oxygen Species
- Troponin I
- Matrix Metalloproteinase 2
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Topics |
- Animals
- Coronary Circulation
(radiation effects)
- Electromagnetic Fields
- Humans
- Interleukin-6
(metabolism)
- Matrix Metalloproteinase 2
(metabolism)
- Myocardial Contraction
(physiology, radiation effects)
- Myocardial Reperfusion Injury
(metabolism, physiopathology, therapy)
- Myocardium
(pathology)
- Organ Culture Techniques
- Rats
- Reactive Oxygen Species
(metabolism)
- Troponin I
(metabolism)
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