Abstract | BACKGROUND: METHODS: OPN-c expression was assessed by immunohistochemistry in 63 ESCC samples and correlated with clinicopathologic factors. Expression was also examined in peripheral blood mononuclear cells (PBMCs) from 120 ESCC patients and 30 healthy subjects. The role of OPN-c mRNA as a tumor marker was investigated by receiver operating characteristic curve (ROC) analysis. RESULTS: Immunohistochemistry showed that OPN-c was expressed in 30 of 63 cancer lesions (48%)and significantly associated with pathological T stage (P=0.038) and overall stage (P=0.023). Real time PCR showed that OPN-c mRNA was expressed at higher levels in the PBMCs of ESCC patients than in those of healthy subjects (P<0.0001) with a sensitivity as an ESCC biomarker of 86.7%. CONCLUSION: Our findings suggest that expression of OPN-c is significantly elevated in ESCCs and this upregulation could be a potential diagnostic marker.
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Authors | Mei-Xiang Zhang, Yi-Jun Xu, Ming-Chen Zhu, Feng Yan |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 14
Issue 12
Pg. 7315-9
( 2013)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 24460294
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- RNA, Messenger
- Osteopontin
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Topics |
- Adult
- Aged
- Biomarkers, Tumor
(genetics, metabolism)
- Carcinoma, Squamous Cell
(genetics, metabolism, pathology)
- Case-Control Studies
- Esophageal Neoplasms
(genetics, metabolism, pathology)
- Female
- Follow-Up Studies
- Humans
- Immunoenzyme Techniques
- Leukocytes, Mononuclear
(metabolism)
- Male
- Middle Aged
- Neoplasm Staging
- Osteopontin
(genetics, metabolism)
- Prognosis
- RNA, Messenger
(genetics)
- ROC Curve
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
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