Elevated serum IgE against MGL_1304 in patients with atopic dermatitis and cholinergic urticaria.

Abstract

BACKGROUND:

MGL_1304 secreted by Malassezia globosa is contained in human sweat and induces histamine release from basophils in patients with atopic dermatitis (AD) at a high positive rate. The aims of this study were to establish the enzyme-linked immunosorbent assay (ELISA) measuring specific immunoglobulins against MGL_1304 and to investigate the levels of these immunoglobulins in sera of patients with various allergic diseases.

METHODS:

Purified MGL_1304 from human sweat (QRX) and recombinant MGL_1304 (rMGL_1304) were prepared for ELISA. To quantify the amount of MGL_1304-specific immunoglobulins, the standard serum was created by pooling sera of 20 patients with AD whose basophils released histamine in response to QRX. A monoclonal antibody which exhibited the highest neutralizing ability against QRX was established as Smith-2, and used as a capture antibody for the assay of QRX-specific IgE. A total of 156 subjects [normal controls (n = 23), AD (n = 63), cholinergic urticaria (CU) (n = 24), bronchial asthma (n = 32), and allergic rhinitis (n = 14)] were enrolled in this study.

RESULTS:

ELISA methods to quantify the specific IgE, IgG and IgG4 against MGL_1304 in sera were successfully established. Levels of QRX-specific IgE in sera of patients with AD and CU were significantly higher than those of normal controls. Moreover, the levels of QRX-specific IgE and rMGL_1304-specific IgE in patients with AD were significantly correlated with their disease severities.

CONCLUSIONS:

These ELISA methods to quantify the specific immunoglobulins against MGL_1304 are easy and useful means to assess allergy to MGL_1304. MGL_1304 contained in sweat is an important antigen for patients with AD and CU.

• Authors: Makiko Hiragun, Takaaki Hiragun, Kaori Ishii, Hidenori Suzuki, Akio Tanaka, Yuhki Yanase, Shoji Mihara, Yoshinori Haruta, Nobuoki Kohno, Michihiro Hide
• Journal: Allergology international : official journal of the Japanese Society of Allergology (Allergol Int) Vol. 63 Issue 1 Pg. 83-93 (Mar 2014) ISSN: 1440-1592 [Electronic] England
• PMID: 24457815 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Immunoglobulin G
• Immunoglobulin E

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Allergens (immunology)
• Antibody Specificity (immunology)
• Basophils (immunology)
• Child
• Child, Preschool
• Dermatitis, Atopic (blood, diagnosis, immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Histamine Release
• Humans
• Immunoglobulin E (blood, immunology)
• Immunoglobulin G (blood, immunology)
• Infant
• Infant, Newborn
• Male
• Middle Aged
• Severity of Illness Index
• Sweat (immunology)
• Urticaria (blood, diagnosis, immunology)
• Young Adult