Abstract | OBJECTIVES: METHODS: In order to evaluate the effects of CHD on the cytotoxicity induced from hypoxia or H/R condition, lactate dehydrogenase (LDH) assay was performed. To explore whether the suppression of neural damage when pre-treated with CHD is associated with its anti-apoptotic effect, the CHD effect on the expression of Bcl-2 and Bax was analyzed by Western blotting analysis. RESULTS: Cytotoxicity of N2a cell line was slightly increased in 42 h hypoxia condition and dramatically increased under the H/R condition. CHD treatment markedly decreased the cytotoxicity in both conditions (P<0.01, P<0.05). H/R markedly increased the expression of the pro-apoptotic protein, Bax, but slightly increased the expression of the anti-apoptotic protein, Bcl-2, compared with the normoxia or hypoxia group. CHD significantly decreased Bax expression (P<0.01) and slightly decreased Bcl-2 expression (P>0.05), resulted in a reduction of Bax/Bcl-2 ratio in N2a cells subjected to H/R. CONCLUSION:
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Authors | Chang-Nam Ko, In-Seo Park, Seong-Uk Park, Woo-Sang Jung, Sang-Kwan Moon, Jung-Mi Park, Chulhun Kang, Ki-Ho Cho |
Journal | Chinese journal of integrative medicine
(Chin J Integr Med)
Vol. 19
Issue 12
Pg. 940-4
(Dec 2013)
ISSN: 1672-0415 [Print] China |
PMID | 24307315
(Publication Type: Journal Article)
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Chemical References |
- Drugs, Chinese Herbal
- Neuroprotective Agents
- Qing Ye Dan
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Topics |
- Animals
- Cell Line, Tumor
- Drugs, Chinese Herbal
(pharmacology)
- Hypoxia
(prevention & control)
- Mice
- Neuroblastoma
(metabolism, pathology)
- Neuroprotective Agents
(pharmacology)
- Reperfusion Injury
(prevention & control)
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