Abstract | OBJECTIVE: METHODS: We studied 35 Wistar rats divided into groups according to reperfusion time. The superior mesenteric artery was clamped for 30 minutes, and blood and biopsies were collected at 1, 3, 6, 12, 24, and 48 hours after reperfusion. We plotted the mean ± standard deviation and compared the baseline and maximum values for each marker using Student's t-test. RESULTS: CONCLUSION: For the evaluation of injury secondary to acute intestinal ischemia reperfusion with a 30 minute ischemia period, we recommend performing histopathological grading, quantification of D- lactate, which is synthesized by intestinal bacteria and is considered an indicator of mucosal injury, and quantification of tumor necrosis factor alpha as indicators of acute inflammation three hours after reperfusion.
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Authors | Francisco Javier Guzmán-de la Garza, Juan Manuel Ibarra-Hernández, Paula Cordero-Pérez, Pablo Villegas-Quintero, Claudia Ivette Villarreal-Ovalle, Liliana Torres-González, Norma Edith Oliva-Sosa, Gabriela Alarcón-Galván, Nancy Esthela Fernández-Garza, Linda Elsa Muñoz-Espinosa, Carlos Rodrigo Cámara-Lemarroy, José Gerardo Carrillo-Arriaga |
Journal | Clinics (Sao Paulo, Brazil)
(Clinics (Sao Paulo))
Vol. 68
Issue 7
Pg. 1034-8
(Jul 2013)
ISSN: 1980-5322 [Electronic] United States |
PMID | 23917671
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Cytokines
- Fatty Acid-Binding Proteins
- Lactate Dehydrogenases
- Aspartate Aminotransferases
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Topics |
- Animals
- Aspartate Aminotransferases
(blood)
- Biomarkers
(blood)
- Biopsy
- Cytokines
(blood)
- Disease Models, Animal
- Fatty Acid-Binding Proteins
(blood)
- Female
- Intestines
(blood supply, pathology)
- Lactate Dehydrogenases
(blood)
- Rats
- Rats, Wistar
- Reference Values
- Reperfusion Injury
(blood)
- Time Factors
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