Acting via its receptor UNC5B,
netrin-1, one of the major neuronal guidance cues, plays an important role in angiogenesis, neurogenesis, tissue morphogenesis, embryonic development,
cancer,
inflammation, and various pathologies. However, its role has not been reported in prostate
carcinoma. To investigate the association of
netrin-1 and UNC5B expression with prostate
carcinoma, several human prostate
carcinoma cell lines were cultured and the expression levels of
netrin-1 and UNC5B were determined by real-time PCR and Western blot.
Calphostin C, (the inhibitor of PKC α) and
phorbol-12-myristate 13-acetate-PMA (the agonist of PKC α) were used to treat the prostate
carcinoma cells, and the cell proliferation and invasion abilities were measured by
CCK-8 and wound-healing assays. Proliferation of DU145 cells was affected by the recruitment of PMA and
calphostin C in a dose-dependent manner. By immunofluorescence, we identified the localization of
netrin-1 and UNC5B in prostate
carcinoma cell lines (DU145, 22RV1, PC3, PC3M, and RWEP) and found that
netrin-1 was highly expressed in the nucleolus but there was no expression of UNC5B. The co-localization expression of PKC α and UNC5B was confirmed by the confocal immunofluorescence. Higher
netrin-1 and lower UNC5B expression in all prostate
carcinoma cell lines indicated that
netrin-1 and UNC5B could be used to predict
metastasis.