In vivo and in vitro anti-inflammatory potential of pentahydroxy-pregn-14-ol, 20-one-β-D-thevetopyranoside in rats.

Abstract

Polyhydroxy pregnane glycoside (PPG), a steroidal glycoside was isolated from Wattakaka volubilis Linn. (Stap.f.). PPG was evaluated for in vivo and in vitro anti-inflammatory activity using acute inflammation and chronic model of inflammation in rats and LPS-induced RAW 264.7 macrophage cells. PPG seemed to be responsible for the anti-inflammatory activity in the studied models. PPG at dose level of both 5 and 10 mg/kg significantly reduced the edema induced by the carrageenan in acute model of inflammation. It also showed significant anti-proliferative effect (dry pellet weight basis) in chronic model of inflammation. Cellular content of granuloma was measured by assaying activity of N-acetyl glucosaminidase (NAG) and total nucleic acid content. PPG at 5 and 10 mg/kg significantly suppressed the cellular infiltration measured by total nucleic acid content. In contrast, NAG activity decreased over a period of 10 days resulting in inhibition of granuloma weight gain. PPG had a more effective response than the reference drug diclofenac sodium in both the models of inflammation. Wattakaka volubilis steroidal glycoside mixture (WVSM) and PPG (1-50 μM) significantly inhibited the COX-2 and iNOS enzymes resulting in low levels of PGE2 and NO in LPS-induced RAW 264.7 macrophage cells. Hence the study supports the traditional use of Wattakaka volubilis and its constituent PPG in treatment of inflammatory disorders.

• Authors: Ram S Jadhav, Md Liyakat Ahmed, Paramjyoti L Swamy, Syed Sanaullah
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 20 Issue 8-9 Pg. 719-22 (Jun 15 2013) ISSN: 1618-095X [Electronic] Germany
• PMID: 23570999 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier GmbH. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Glycosides
• Lipopolysaccharides
• Pregnanes
• pregnane glycoside
• Nitric Oxide
• Carrageenan
• Pregnanolone
• Nitric Oxide Synthase Type II
• Nos2 protein, rat
• Cyclooxygenase 2
• Ptgs2 protein, rat
• Hexosaminidases
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, isolation & purification, pharmacology)
• Apocynaceae (chemistry)
• Carrageenan (adverse effects)
• Cell Line
• Cyclooxygenase 2 (metabolism)
• Dinoprostone (metabolism)
• Disease Models, Animal
• Edema (chemically induced, drug therapy)
• Female
• Glycosides (chemistry, isolation & purification, pharmacology)
• Granuloma (drug therapy)
• Hexosaminidases (metabolism)
• Lipopolysaccharides (adverse effects)
• Macrophages (drug effects)
• Male
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Plants, Medicinal (chemistry)
• Pregnanes (chemistry, isolation & purification, pharmacology)
• Pregnanolone (analogs & derivatives, chemistry, isolation & purification, pharmacology)
• Rats