Abstract | OBJECTIVE: METHODS: RESULTS: Results showed that PD administration caused a remarkable reduction in proliferation of A375 cells, without showing much cytotoxicity on peripheral blood mononuclear cells. Generation of ROS and DNA damage, which made the cancer cells prone to apoptosis, were found to be enhanced in PD-treated cells. These results were duly supported by the analytical data on expression of different cellular and nuclear proteins, as for example, by down-regulation of Akt and Bcl-2, up-regulation of p53, Bax and caspase 3, and an increase in number of cell deaths by apoptosis in A375 cells. CONCLUSION: Overall results demonstrate anticancer potentials of PD on A375 cells through activation of caspase-mediated signaling and ROS generation.
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Authors | Samrat Ghosh, Kausik Bishayee, Avijit Paul, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh |
Journal | Journal of integrative medicine
(J Integr Med)
Vol. 11
Issue 2
Pg. 116-24
(Mar 2013)
ISSN: 2095-4964 [Print] Netherlands |
PMID | 23506692
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Drugs, Chinese Herbal
- Proto-Oncogene Proteins c-bcl-2
- Reactive Oxygen Species
- Caspase 3
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Apoptosis
(drug effects)
- Caspase 3
(genetics, metabolism)
- Cell Line, Tumor
- Drugs, Chinese Herbal
(pharmacology)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Melanoma
(drug therapy, genetics, metabolism, physiopathology)
- Phytolacca
(chemistry)
- Phytotherapy
- Proto-Oncogene Proteins c-bcl-2
(genetics, metabolism)
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
(drug effects)
- Skin Neoplasms
(drug therapy, genetics, metabolism, physiopathology)
- Up-Regulation
(drug effects)
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