Fel d 1-airway inflammation prevention and treatment by co-immunization vaccine via induction of CD4+CD25-Foxp3+ Treg cells.

Abstract

Pet allergens are major causes for asthma and allergic rhinitis. Fel d 1 protein, a key pet allergen from domestic cat, can sensitize host and trigger asthma attack. In this study, we report that co-immunization with recombinant Fel d 1 protein (rFel d 1) plus plasmid DNA that contains Fe1 d 1 gene was effective in preventing and treating the natural Fel d 1 (nFel d 1) induced allergic airway inflammation in mice. A population of T regulatory cells (iTreg) exhibiting a CD4+CD25-Foxp3+ phenotype and expressing IL-10 and TGF-β was induced by this co-immunization strategy. Furthermore, after adoptive transfers of the iTreg cells, mice that were pre-sensitized and challenged with nFel d 1 exhibited less signs of allergic inflammation, AHR and a reduced allergic immune response. These data indicate that co-immunization with DNA and protein mixture vaccine may be an effective treatment for cat allergy.

• Authors: Yechun Pei, Shuang Geng, Lin Liu, Fengxiang Yan, Hong Guan, Jian Hou, Yongfu Chen, Bin Wang, Xiaorong An
• Journal: Human vaccines & immunotherapeutics (Hum Vaccin Immunother) Vol. 9 Issue 5 Pg. 1019-31 (May 2013) ISSN: 2164-554X [Electronic] United States
• PMID: 23324570 (Publication Type: Journal Article)

Chemical References

• Forkhead Transcription Factors
• Foxp3 protein, mouse
• Glycoproteins
• Il2ra protein, mouse
• Interleukin-2 Receptor alpha Subunit
• Vaccines, DNA
• Fel d 1 protein, Felis domesticus

Topics

• Animals
• Asthma (prevention & control, therapy)
• CD4-Positive T-Lymphocytes (chemistry, immunology)
• Cats
• Disease Models, Animal
• Female
• Forkhead Transcription Factors (analysis)
• Glycoproteins (administration & dosage, immunology)
• Interleukin-2 Receptor alpha Subunit (analysis)
• Mice
• Mice, Inbred BALB C
• Rhinitis (prevention & control, therapy)
• T-Lymphocytes, Regulatory (chemistry, immunology)
• Treatment Outcome
• Vaccination (methods)
• Vaccines, DNA (administration & dosage, immunology)