KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties.
Atopic dermatitis (AD) is an inflammatory
skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to
allergens that results in elevated serum eosinophil and
Immunoglobulin E (
IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329
ethanol extract on the
ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (
DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum
IgE, and expression of
cytokine mRNA in the
atopic dermatitis skin lesions of OVA
allergen- or
DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or
DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions,
mRNA expression of interleukine (IL)-4,
IL-13, and
IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum
IgE level in both the OVA- and
DNCB-induced
atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.