Abstract |
Azadirachta indica Linn. (Meliaceae) has been used from ancient times as a remedy for various ailments. The present study was designed to investigate the antioxidant and anti-apoptotic properties of A. indica seed extract (ASE) in transient middle cerebral artery occlusion (MCAO) rat model. Antioxidant potential of ASE was determined in vitro. Further, ASE was evaluated against neurological deficits, histological alterations (TTC, CV and H&E) and oxidative damage ( TBARS, GSH and nitrite) in MCAO rats. Moreover, caspase-3 and -9 were analyzed to evaluate the anti-apoptotic activity of ASE. ASE has shown potent in vitro reducing power (126.2 mg AsAE/g extract) and free radical scavenging activities (DPPH 171.0 and NO 176.0 μg/ml). Furthermore, ASE inhibited oxidative stress and decreased the activities of caspase-3 (26.7 %, p < 0.05) and caspase-9 (31.2 %, p < 0.01) thus, reduced neuronal loss in MCAO rats. Our data revealed that ASE has potent antioxidant and anti-apoptotic properties, and may be explored for its active constituents against neurodegenerative diseases.
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Authors | Kumar Vaibhav, Pallavi Shrivastava, Andleeb Khan, Hayate Javed, Rizwana Tabassum, Md Ejaz Ahmed, M Badruzzaman Khan, Mohd Moshahid Khan, Farah Islam, Sayeed Ahmad, M Saeed Siddiqui, Mohammed M Safhi, Fakhrul Islam |
Journal | Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
(Neurol Sci)
Vol. 34
Issue 8
Pg. 1321-30
(Aug 2013)
ISSN: 1590-3478 [Electronic] Italy |
PMID | 23187787
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Apoptosis
(drug effects)
- Azadirachta
- Brain
(drug effects, pathology)
- Brain Ischemia
(drug therapy, pathology)
- Disease Models, Animal
- Male
- Oxidative Stress
(drug effects)
- Phytotherapy
- Plant Extracts
(therapeutic use)
- Psychomotor Disorders
(drug therapy)
- Rats
- Rats, Wistar
- Reperfusion Injury
(drug therapy, pathology)
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