RIP140 is associated with subclinical inflammation in type 2 diabetic patients.

Abstract

AIMS:

To evaluate the expression level of RIP140 (receptor interaction protein 140) and its correlation with inflammatory cytokine production and free fatty acids (FFAs) in type 2 diabetes.

METHODS:

Plasma and peripheral blood mononuclear cells (PBMCs) were collected from 24 diabetic and 30 healthy individuals. The levels of FFAs, TC, TG, HDL-C, LDL-C, FIN, and FBG were measured. The insulin resistance index was calculated using the homeostasis model assessment (HOMA). Additionally, PBMCs from control group were cultured alone or with 500 μmol/L palmitic acid (PA). Levels of RIP140 TNF-α, and IL-6 in PBMCs were analyzed using real-time RT-PCR, Western blots and ELISA. The relationship between RIP140 and other variables was performed using SPSS 11.5 software.

RESULTS:

TG, LDL-C, FIN, FBG, HOMA, and HDL-C were significantly different between diabetic patients and the control group. Levels of RIP140, TNF-α, and IL-6 were higher in the diabetic group compared to control. RIP140 expression was positively correlated with FFAs, HDL-c, TNF-α, IL-6, FIN, FBG, and HOMA. Finally, 500 μmol/L PA treatment increased RIP140 expression and the secretion of inflammatory cytokines in cultured control PBMCs.

CONCLUSIONS:

Increased RIP140 level may be closely associated with inflammation and disorder of lipid and glucose metabolism in diabetic patients.

• Authors: J Xue, H Zhao, G Shang, R Zou, Z Dai, D Zhou, Q Huang, Y Xu
• Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association (Exp Clin Endocrinol Diabetes) Vol. 121 Issue 1 Pg. 37-42 (Jan 2013) ISSN: 1439-3646 [Electronic] Germany
• PMID: 22956256 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Adaptor Proteins, Signal Transducing
• IL6 protein, human
• Interleukin-6
• Lipids
• NRIP1 protein, human
• Nuclear Proteins
• Nuclear Receptor Interacting Protein 1
• Tumor Necrosis Factor-alpha
• Glucose

Topics

• Adaptor Proteins, Signal Transducing (biosynthesis)
• Adult
• Cells, Cultured
• Diabetes Mellitus, Type 2 (blood, pathology)
• Female
• Gene Expression Regulation
• Glucose (metabolism)
• Humans
• Inflammation (metabolism, pathology)
• Interleukin-6 (blood)
• Leukocytes, Mononuclear (metabolism, pathology)
• Lipid Metabolism
• Lipids (blood)
• Male
• Middle Aged
• Nuclear Proteins (biosynthesis)
• Nuclear Receptor Interacting Protein 1
• Tumor Necrosis Factor-alpha (blood)