Prostaglandins mediate the fetal pulmonary response to intrauterine inflammation.

Abstract

Intra-amniotic (IA) lipopolysaccharide (LPS) induces intrauterine and fetal lung inflammation and increases lung surfactant and compliance in preterm sheep; however, the mechanisms are unknown. Prostaglandins (PGs) are inflammatory mediators, and PGE(2) has established roles in fetal lung surfactant production. The aim of our first study was to determine PGE(2) concentrations in response to IA LPS and pulmonary gene expression for PG synthetic [prostaglandin H synthase-2 (PGHS-2) and PGE synthase (PGES)] and PG-metabolizing [prostaglandin dehydrogenase (PGDH)] enzymes and PGE(2) receptors. Our second study aimed to block LPS-induced increases in PGE(2) with a PGHS-2 inhibitor (nimesulide) and determine lung inflammation and surfactant protein mRNA expression. Pregnant ewes received an IA saline or LPS injection at 118 days of gestation. In study 1, fetal plasma and amniotic fluid were sampled before and at 2, 4, 6, 12, and 24 h after injection and then daily, and fetuses were delivered 2 or 7 days later. Amniotic fluid PGE(2) concentrations increased (P < 0.05) 12 h and 3-6 days after LPS. Fetal lung PGHS-2 mRNA and PGES mRNA increased 2 (P = 0.0084) and 7 (P = 0.014) days after LPS, respectively. In study 2, maternal intravenous nimesulide or vehicle infusion began immediately before LPS or saline injection and continued until delivery 2 days later. Nimesulide inhibited LPS-induced increases in PGE(2) and decreased fetal lung IL-1β and IL-8 mRNA (P ≤ 0.002) without altering lung inflammatory cell infiltration. Nimesulide decreased surfactant protein (SP)-A (P = 0.05), -B (P = 0.05), and -D (P = 0.0015) but increased SP-C mRNA (P = 0.023). Thus PGHS-2 mediates, at least in part, fetal pulmonary responses to inflammation.

• Authors: Alana J Westover, Stuart B Hooper, Megan J Wallace, Timothy J M Moss
• Journal: American journal of physiology. Lung cellular and molecular physiology (Am J Physiol Lung Cell Mol Physiol) Vol. 302 Issue 7 Pg. L664-78 (Apr 01 2012) ISSN: 1522-1504 [Electronic] United States
• PMID: 22287609 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclooxygenase 2 Inhibitors
• Interleukin-1beta
• Interleukin-8
• Lipopolysaccharides
• Pulmonary Surfactant-Associated Proteins
• Pulmonary Surfactants
• RNA, Messenger
• Sulfonamides
• Cyclooxygenase 2
• Dinoprostone
• nimesulide

Topics

• Amniotic Fluid (chemistry)
• Animals
• Chorioamnionitis (immunology, metabolism)
• Cyclooxygenase 2 (metabolism)
• Cyclooxygenase 2 Inhibitors (pharmacology)
• Dinoprostone (blood, metabolism)
• Female
• Interleukin-1beta (biosynthesis, genetics)
• Interleukin-8 (biosynthesis, genetics)
• Lipopolysaccharides (immunology)
• Lung (embryology, metabolism, pathology, physiopathology)
• Lung Compliance
• Pneumonia (embryology, immunology, metabolism, physiopathology)
• Pregnancy
• Pulmonary Surfactant-Associated Proteins (biosynthesis)
• Pulmonary Surfactants (metabolism)
• RNA, Messenger (genetics, metabolism)
• Random Allocation
• Sheep
• Sulfonamides (pharmacology)
• Uterus (immunology)