Coordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping.

Abstract

Asthma is a chronic allergic disorder characterised by chronic inflammation. The balance of type I and type II (CD4+) T helper cells is of critical importance. In asthma there is an overexpression of T(H)2 cytokines, such as IL-4, IL-5 and IL-13. The genes encoding these cytokines are located together the same chromosomal region, 5q31.1 in humans. Here we confirm a central role for the transcription factors NFAT and GATA3 in the regulation of human IL-4 and IL-13. Chromatin Conformation Capture (3C) demonstrated the formation of specific ligation products containing spliced IL-4 and IL-13 DNA sequences in human T(H)2 polarised HuT-78 cells. This suggests that co-ordinate expression of T(H)2 cytokines, under the control of GATA3 and NFAT1 is due to the formation of a chromatin hub by DNA looping.

• Authors: Xin Yao, Wangjian Zha, Wei Song, Haiyan He, Mao Huang, Elen Jazrawi, Paul Lavender, Peter J Barnes, Ian M Adcock, Andrew L Durham
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 417 Issue 3 Pg. 996-1001 (Jan 20 2012) ISSN: 1090-2104 [Electronic] United States
• PMID: 22226971 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• CD28 Antigens
• Chromatin
• GATA3 Transcription Factor
• GATA3 protein, human
• Interleukin-13
• NFATC Transcription Factors
• NFATC2 protein, human
• Receptors, IgE
• Interleukin-4
• DNA

Topics

• Asthma (immunology)
• Base Sequence
• CD28 Antigens (immunology)
• Cell Line
• Cell Nucleus (metabolism)
• Chromatin (chemistry, ultrastructure)
• DNA (chemistry, genetics)
• GATA3 Transcription Factor (metabolism)
• Gene Expression Regulation
• Humans
• Immunoprecipitation
• Interleukin-13 (genetics)
• Interleukin-4 (genetics)
• Lymphocyte Activation (genetics)
• Molecular Sequence Data
• NFATC Transcription Factors (metabolism)
• Nucleic Acid Conformation
• Receptors, IgE (immunology)
• Th2 Cells (immunology)