ETS-GS, a new antioxidant, ameliorates renal ischemia-reperfusion injury in a rodent model.

Abstract

BACKGROUND:

Ischemia-reperfusion (I/R) contributes to acute kidney injury (AKI). On the other hand, anti-oxidative drugs help to prevent renal injury caused by I/R. The current study examined whether a new antioxidant, ETS-GS, inhibits reactive oxygen species (ROS) generation and thereby prevents renal I/R injury in rodent models.

METHODS:

Rats with experimentally-induced renal I/R injury were treated concurrently with an intravenous injection of either ETS-GS or saline. Anesthesia was induced with sevoflurane.

RESULTS:

Histologic examination revealed marked reduction of interstitial congestion, edema, inflammation, and hemorrhage in kidney tissue harvested 24 h after ETS-GS treatment. Renal I/R-induced secretion of nitric oxide (NO) in serum was inhibited by ETS-GS treatment. Furthermore, malondialdehyde (MDA) levels in the kidney were significantly lower in ETS-GS-treated rats with renal I/R. Moreover, when murine macrophage-like RAW264.7 cells were stimulated with antimycin A in the presence or absence of simultaneous ETS-GS treatment, ETS-GS decreased ROS levels.

CONCLUSIONS:

Thus, ETS-GS lowered ROS levels in cultured cells, reduced serum NO levels, decreased renal MDA levels, and protected rats against I/R-induced kidney injury. Given these in vitro and in vivo findings, ETS-GS is a strong candidate for future exploration of therapeutic potential in various human I/R diseases.

• Authors: Satoshi Hagiwara, Hironori Koga, Hideo Iwasaka, Kyosuke Kudo, Akira Hasegawa, Jyunya Kusaka, Isao Yokoi, Takayuki Noguchi
• Journal: The Journal of surgical research (J Surg Res) Vol. 171 Issue 1 Pg. 226-33 (Nov 2011) ISSN: 1095-8673 [Electronic] United States
• PMID: 20451924 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Oligopeptides
• Reactive Oxygen Species
• gamma-L-glutamyl-S-(2-(((3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12- trimethyltridecyl)-2H-1-benzopyran-6-yl)oxy)carbonyl)-3-oxo-3-((2-sulfoethyl)amino)propyl)-L-cysteinylglycine sodium salt
• Nitric Oxide
• Malondialdehyde
• Creatinine

Topics

• Animals
• Antioxidants (chemistry, pharmacology)
• Blood Urea Nitrogen
• Cell Line
• Creatinine (blood)
• Disease Models, Animal
• Kidney (drug effects, metabolism, pathology)
• Kidney Diseases (drug therapy, metabolism, pathology)
• Macrophages (cytology, drug effects)
• Male
• Malondialdehyde (metabolism)
• Mice
• Nitric Oxide (metabolism)
• Oligopeptides (chemistry, pharmacology)
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)
• Reperfusion Injury (drug therapy, metabolism, pathology)
• Survival Rate