In experiments on the anaesthetized dogs with modeling of experimental
ischemia (90 min) and reperfusion (180 min), the participation of biochemical processes in the cardioprotective effect of the preischemic activation of
ATP-sensitive
potassium (
KATP) channels caused by intravenous introduction of
flokalin, a new
fluorine-containing opener of these channels was shown.
Flokalin was introduced in a dose 0.1 mg/kg of animal
body weight which practically did not change the parameters of hemodynamic in normoxia. Thus, the experiments investigating the influence offlokalin on changes of biochemical parameters of arterial blood during
ischemia-reperfusion of myocardium showed certain features of
ischemia-reperfusion syndrome development during stimulation of K(
ATP) channels. The analysis of biochemical parameters of blood showed that
flokalin suppressed
free radical reactions and had
antioxidant properties: reduced quantity of H2O2 and NO3- (the last can interpreted as a reduction in
peroxynitrites formation), prevented the decline of
catalase and
superoxide dismutase activity. Practically constant content of low-molecular nitrosothiols in blood during all duration of experiment and increase of NO2-level during reperfusion may indicate on intact functions of the NO system and protective influence of
flokalin during
ischemia-reperfusion of myocardium. Practically unchanged content of inorganic
phosphorus and
uric acid in blood during
ischemia- reperfusion under conditions of preischemic introduction of
flokalin indicates the prevention of
ATP degradation and fomation of both
superoxide anion by xanthinoxidase and
peroxynitrite by it interaction with
nitric oxide. All mentioned properties of
flokalin related to the changes of biochemical parameters of arterial blood, together with the changes of parameters of hemodynamics, result in diminishment of
infarct size of myocardium after
ischemia-reperfusion by 37% versus control experiments. K(
ATP) channels,
flokalin,
ischemia-reperfusion, free radikaly, NO system.