Abstract | PURPOSE:
RanBP2 is a small ubiquitin-like modifier ligase for DNA topoisomerase II (TopoII) and plays a role in maintaining chromosome stability by recruiting TopoII to centromeres during mitosis. Engineered-mice with low amounts of RanBP2 have been reported to form lung adenocarcinomas. Furthermore, in the murine embryonic fibroblasts, formation of chromatin bridges in anaphase, a distinctive feature of cells with impaired DNA decatenation by chemical inhibition of TopoII, has been reported. In this study, we tested whether the association between mRNA expression of the RanBP2 gene and chemosensitivity of a TopoII inhibitor, amrubicin could be seen. METHODS: Using a panel of 20 lung cancer cell lines, the mRNA expression levels of the RanBP2, TopoII-alpha and TopoII-beta genes were examined by quantitative real-time reverse transcription PCR. The in vitro cytotoxicity of amrubicin was assessed using a tetrazolium-based colorimetric assay (MTT assay). RESULTS: CONCLUSIONS:
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Authors | Yoshitsugu Horio, Hirotaka Osada, Junichi Shimizu, Shizu Ogawa, Toyoaki Hida, Yoshitaka Sekido |
Journal | Cancer chemotherapy and pharmacology
(Cancer Chemother Pharmacol)
Vol. 66
Issue 2
Pg. 237-43
(Jul 2010)
ISSN: 1432-0843 [Electronic] Germany |
PMID | 19809814
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anthracyclines
- Antibiotics, Antineoplastic
- Biomarkers
- Coloring Agents
- Isoenzymes
- Molecular Chaperones
- Nuclear Pore Complex Proteins
- RNA, Messenger
- Tetrazolium Salts
- Thiazoles
- ran-binding protein 2
- amrubicin
- DNA Topoisomerases, Type II
- thiazolyl blue
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Topics |
- Anthracyclines
(toxicity)
- Antibiotics, Antineoplastic
(toxicity)
- Biomarkers
- Blotting, Western
- Cell Line, Tumor
- Cell Survival
- Chromosomal Instability
(drug effects)
- Coloring Agents
- DNA Topoisomerases, Type II
(biosynthesis)
- Humans
- Isoenzymes
(biosynthesis)
- Lung Neoplasms
(drug therapy, metabolism, pathology)
- Molecular Chaperones
(biosynthesis)
- Nuclear Pore Complex Proteins
(biosynthesis)
- RNA, Messenger
(biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
- Tetrazolium Salts
- Thiazoles
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