Expression of semaphorin 5A and its receptor plexin B3 contributes to invasion and metastasis of gastric carcinoma.

Abstract

AIM:

To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.

METHODS:

Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting.

RESULTS:

The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3.

CONCLUSION:

Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.

• Authors: Guo-Qing Pan, Hong-Zheng Ren, Shu-Fang Zhang, Xi-Mei Wang, Ji-Fang Wen
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 15 Issue 22 Pg. 2800-4 (Jun 14 2009) ISSN: 2219-2840 [Electronic] United States
• PMID: 19522033 (Publication Type: Journal Article)

Chemical References

• Membrane Proteins
• Nerve Tissue Proteins
• Neural Cell Adhesion Molecules
• PLXNB3 protein, human
• SEMA5A protein, human
• Semaphorins

Topics

• Aged
• Aged, 80 and over
• Female
• Gastric Mucosa (metabolism, pathology)
• Humans
• Lymphatic Metastasis (pathology)
• Male
• Membrane Proteins (genetics, metabolism)
• Middle Aged
• Neoplasm Invasiveness (pathology)
• Nerve Tissue Proteins (genetics, metabolism)
• Neural Cell Adhesion Molecules (genetics, metabolism)
• Semaphorins
• Stomach Neoplasms (metabolism, pathology)