Abstract | OBJECTIVE: The discovery of IL-7R(alpha) polymorphisms implicated in the pathogenesis of multiple sclerosis has highlighted the importance of interleukin 7 (IL-7) in central nervous system diseases. Hypoxia affects neurological disease states in part by modulating expression of many early and late response genes. The present work used cultured PC12 cells to investigate the effect of hypoxia on IL-7 expression. METHOD: RESULTS: Exposure of PC12 cells to 1% oxygen for 6 hours decreased IL-7 mRNA by 77% using RT-PCR (p<0.01). Exposure to 1% oxygen for 24 hours decreased IL-7 protein in the medium by 21% (p<0.05). As hypoxia duration increased (2, 4, 6 and 24 hours) or oxygen concentrations decreased (10%, 5% and 1%), IL-7 mRNA expression progressively decreased. Removal of extracellular free Ca(2+) completely prevented these hypoxia-induced decreases of IL-7 mRNA. DISCUSSION: Since IL-7 exhibits trophic properties in developing brain, down-regulation of IL-7 by hypoxia may contribute to hypoxia-induced injury to neural cells.
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Authors | Aigang Lu, Joseph F Clark, Ruiqiong Ran, Gail Pyne-Geithman, Kenneth R Wagner, David E Millhorn, Frank R Sharp |
Journal | Neurological research
(Neurol Res)
Vol. 31
Issue 5
Pg. 545-9
(Jun 2009)
ISSN: 0161-6412 [Print] England |
PMID | 19055876
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Interleukin-7
- RNA, Messenger
- Oxygen
- Calcium
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Topics |
- Animals
- Blotting, Western
- Calcium
(pharmacology)
- Cell Hypoxia
(genetics)
- Down-Regulation
- Interleukin-7
(metabolism)
- Oxygen
(pharmacology)
- PC12 Cells
- RNA, Messenger
(metabolism)
- Rats
- Reverse Transcriptase Polymerase Chain Reaction
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