Abstract | CONTEXT: OBJECTIVE: DESIGN:
Glucose metabolism was measured during two hyperinsulinemic [ insulin levels of 100 pmol/liter (low-dose clamp) and 400 pmol/liter (medium-dose clamp)] euglycemic (5 mmol/liter) clamps on two occasions: without or with lipopolysaccharide (LPS). SETTING: The study was conducted at the Academic Medical Center, Metabolic and Clinical Research Unit (Amsterdam, The Netherlands). PARTICIPANTS: Eighteen healthy male volunteers participated in the study. INTERVENTION: A hyperinsulinemic euglycemic (5 mmol/liter) clamp with LPS (two groups of six subjects; insulin infusion at rates of either 10 or 40 mU.m(-2).min(-1)) or without LPS (n = 6; both insulin infusions in same subjects). MAIN OUTCOME MEASURE: RESULTS: Hepatic insulin sensitivity, defined as a decrease in endogenous glucose production during hyperinsulinemia (100 pmol/liter), was higher in the LPS group compared to the control group (P = 0.010). Insulin-stimulated peripheral glucose uptake was higher in both clamps after LPS compared to the control setting (P = 0.006 and 0.010), despite a significant increase in the plasma concentrations of norepinephrine and cytokines in the LPS group during both clamps. CONCLUSIONS:
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Authors | Saskia N van der Crabben, Regje M E Blümer, Michiel E Stegenga, Mariëtte T Ackermans, Erik Endert, Michael W T Tanck, Mireille J Serlie, Tom van der Poll, Hans P Sauerwein |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 94
Issue 2
Pg. 463-8
(Feb 2009)
ISSN: 0021-972X [Print] United States |
PMID | 18984669
(Publication Type: Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Cytokines
- Hormones
- Insulin
- Lipopolysaccharides
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Topics |
- Adult
- Blood Glucose
(drug effects, metabolism)
- Cytokines
(blood)
- Endotoxemia
(blood, chemically induced, metabolism)
- Glucose Clamp Technique
(methods)
- Health
- Hormones
(blood)
- Humans
- Infusion Pumps
- Insulin
(blood, metabolism)
- Insulin Resistance
(physiology)
- Lipopolysaccharides
(administration & dosage, pharmacology)
- Liver
(drug effects, metabolism)
- Male
- Time Factors
- Young Adult
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