Abstract | OBJECTIVE: METHODS: Either LPS (100 ng/mL) or hyperoxia (60%), or a combination of both was employed to stimulate confluent HELFs. After 0.5, 1, 2 and 4 hrs of stimulation, the nuclear translocation of two subunits p50 and p65 in HELFs was detected with immunocytochemistry. Reverse transcription quantitative polymerase chain reaction (RT-PCR) was used to measure mRNA expression of NF-kappaB p50 and p65. RESULTS: LPS or hyperoxia stimulation induced the nuclear translocation of p50 and p65 at 30 minutes of exposure. mRNA expression of NF-kappaB p50 and p65 peaked at 1 hr and then gradually decreased. A stimulation of LPS combined with hyperoxia induced the nuclear translocation of p50 and p65. NF-kappaB p50 and p65 mRNA expression peaked at 2 hrs of stimulation and then decreased slowly, but was significantly higher than that in the LPS or hyperoxia stimulation alone group 4 hrs after stimulation. CONCLUSIONS:
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Authors | Xiao-Ting Zhang, Jian Liu, Xiao Yu, Qin Ning, Xiao-Ping Luo |
Journal | Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
(Zhongguo Dang Dai Er Ke Za Zhi)
Vol. 10
Issue 5
Pg. 661-4
(Oct 2008)
ISSN: 1008-8830 [Print] China |
PMID | 18947494
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipopolysaccharides
- NF-kappa B p50 Subunit
- Transcription Factor RelA
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Topics |
- Bronchopulmonary Dysplasia
(etiology)
- Fibroblasts
(metabolism)
- Humans
- Hyperoxia
(metabolism)
- Immunohistochemistry
- Infant, Newborn
- Lipopolysaccharides
(toxicity)
- Lung
(cytology, embryology)
- NF-kappa B p50 Subunit
(analysis, genetics, metabolism)
- Transcription Factor RelA
(analysis, genetics, metabolism)
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