Abstract | BACKGROUND AND OBJECTIVE:
Cytotoxic T-lymphocyte antigen 4 (CTLA-4) signalling of regulatory T cells regulates mucosal lymphocyte tolerance and differentiation, and may therefore have a beneficial effect in allergic diseases such as asthma. The aim of this study was to evaluate the effects of fluticasone propionate (FP) on CD4+CD25+ T cell co-expression of CTLA-4 in the sputum of mild asthmatic subjects. METHODS: RESULTS: FP treatment increased co-expression of CTLA-4 on sputum CD4+CD25+ cells from a mean (SEM) of 7.9% (1.8) to 12.7% (3.3) after 14 days treatment (P < 0.05) compared with placebo. FP treatment also significantly increased IL-10 levels, reduced per cent sputum eosinophils, and reduced airway hyperresponsiveness (P < 0.05). There was a significant negative correlation between the change in airway hyperresponsiveness and per cent sputum eosinophils (P < 0.01), but no correlation with changes in CTLA-4+CD4+CD25+ cells (P > 0.05). There was no change in the levels of sputum IL-13 or TGF-beta. CONCLUSIONS: The percentage of airway CTLA-4+CD4+CD25+ cells increased after FP treatment, coincident with improvements in airway inflammation and hyperresponsiveness.Whether improved asthma assessments are related to the increase in CTLA-4+CD4+CD25+ cells and thus improved regulation of T-cell tolerance and differentiation will require a larger sample size to determine. The normalization of CTLA-4+CD4+CD25+ cells in asthma may contribute to the management of this disease.
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Authors | Tomotaka Kawayama, Takashi Kinoshita, Haruki Imaoka, Gail M Gauvreau, Paul M O'Byrne, Hisamichi Aizawa |
Journal | Respirology (Carlton, Vic.)
(Respirology)
Vol. 13
Issue 7
Pg. 1000-1007
(Nov 2008)
ISSN: 1440-1843 [Electronic] Australia |
PMID | 18699801
(Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial)
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Chemical References |
- Androstadienes
- Antigens, CD
- Bronchodilator Agents
- CTLA-4 Antigen
- CTLA4 protein, human
- Interleukin-2 Receptor alpha Subunit
- Fluticasone
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Topics |
- Administration, Inhalation
- Adult
- Androstadienes
(administration & dosage)
- Antigens, CD
(biosynthesis, drug effects, immunology)
- Asthma
(drug therapy, immunology, pathology)
- Bronchodilator Agents
(administration & dosage)
- CD4-Positive T-Lymphocytes
(drug effects, immunology)
- CTLA-4 Antigen
- Cross-Over Studies
- Dose-Response Relationship, Drug
- Double-Blind Method
- Enzyme-Linked Immunosorbent Assay
- Female
- Flow Cytometry
- Fluticasone
- Follow-Up Studies
- Humans
- Interleukin-2 Receptor alpha Subunit
(immunology)
- Lymphocyte Count
- Male
- Severity of Illness Index
- Sputum
(chemistry, cytology)
- Young Adult
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