Abstract | BACKGROUND/AIMS: RESULTS: CO lowered renal AdoHcy- hydrolase mRNA expression by 64% whereas AdoHcy- hydrolase activity was not changed during 4h of CO exposure 0.7+/-0.04 mU/mg (control) vs. 0.75+/-0.06 mU/mg protein. Using two-channel immunofluorescence confocal laser scanning microscope AdoHcy- hydrolase was visualized in different cells of the hypoxic rat kidney. A very bright immunofluorescence of AdoHcy- hydrolase was observed in the nuclei of single interstitial cells of renal cortex and outer medulla which respond to hypoxia with increased EPO secretion indicating translocation of AdoHcy- hydrolase from the cytosol to the nucleus. CONCLUSIONS: These data suggest that AdoHcy- hydrolase accumulation in the nucleus of adult mammalian cells is involved in maintaining efficient transmethylation reactions in transcriptionally active cells by removing the product inhibitor AdoHcy.
|
Authors | Doris Kloor, Marina Hermes, Katrin Fink, Heide Schmid, Karin Klingel, Andreas Mack, Almut Grenz, Hartmut Osswald |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 19
Issue 1-4
Pg. 57-66
( 2007)
ISSN: 1015-8987 [Print] Germany |
PMID | 17310100
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 2007 S. Karger AG, Basel. |
Chemical References |
- Erythropoietin
- Carbon Monoxide
- Adenosylhomocysteinase
|
Topics |
- Adenosylhomocysteinase
(metabolism)
- Animals
- Carbon Monoxide
(pharmacology)
- Cell Nucleus
(metabolism)
- Erythropoietin
(blood, metabolism)
- Hypoxia
- Immunohistochemistry
- In Situ Hybridization
- Kidney
(drug effects, enzymology)
- Male
- Methylation
- Rats
- Rats, Sprague-Dawley
- Transcription, Genetic
|