Abstract | BACKGROUND: METHODS: RESULTS: Severity of pancreatitis and cytokine gene expression in peripheral blood mononuclear cells (PBMC) were evaluated. Administration of AS-PAP, but not the scrambled oligodeoxyribonucleotide (SC-PAP) control, reduced pancreatitis-induced PAP expression by 55.2 +/- 6.4%, 44.0 +/- 8.9%, and 38.9 +/- 10.7% for PAP isoforms I, II, and III, respectively, compared to saline-treated controls (P < 0.05 for all). Inhibition of PAP expression significantly worsened pancreatitis: serum amylase activity, pancreas wet weight (reflecting edema), and serum C-reactive protein levels all increased in AS-PAP-treated animals compared to SC-PAP-treated controls (by 3.5-, 1.7-, and 1.7-fold, respectively; P < 0.05 for all). Histopathologic evaluation of pancreas revealed worsened edema, elevated leukocyte infiltration, and fat necrosis after AS-PAP treatment. Gene expressions of IL-1 microm and IL-4 were significantly higher in PBMC isolated from AS-PAP-treated rats compared to SC-PAP controls. CONCLUSION: This is the first in vivo evidence indicating that PAP mediates significant protection against pancreatic injury. Our data suggest that PAP may exert its protective function by suppressing local pancreatic as well as systemic inflammation during acute pancreatitis.
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Authors | H Zhang, E Kandil, Y-Y Lin, G Levi, M E Zenilman |
Journal | Scandinavian journal of gastroenterology
(Scand J Gastroenterol)
Vol. 39
Issue 9
Pg. 870-81
(Sep 2004)
ISSN: 0036-5521 [Print] England |
PMID | 15513386
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Neoplasm
- Biomarkers, Tumor
- DNA, Antisense
- Genetic Markers
- Lectins, C-Type
- Oligonucleotides
- Pancreatitis-Associated Proteins
- REG3A protein, human
- RNA, Messenger
- Reg3b protein, rat
- C-Reactive Protein
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Topics |
- Acute Disease
- Analysis of Variance
- Animals
- Antigens, Neoplasm
(genetics, metabolism)
- Base Sequence
- Biomarkers, Tumor
(genetics, metabolism)
- C-Reactive Protein
- DNA, Antisense
(pharmacology)
- Disease Models, Animal
- Female
- Gene Expression Regulation
(drug effects)
- Genetic Markers
(genetics)
- Lectins, C-Type
(genetics, metabolism)
- Male
- Molecular Sequence Data
- Oligonucleotides
(pharmacology)
- Pancreatitis
(drug therapy, genetics, physiopathology)
- Pancreatitis-Associated Proteins
- Probability
- Prognosis
- RNA, Messenger
(analysis)
- Rats
- Rats, Sprague-Dawley
- Reference Values
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Treatment Outcome
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