Abstract | OBJECTIVE: To investigate the effect of a single intravenous treatment with glucocorticoids (GC) encapsulated in long-circulating PEG- liposomes on both joint inflammation and cartilage destruction and to investigate the phenomenon of selective homing of these liposomes in the inflamed synovium. METHODS: RESULTS: Treatment of CIA with 10 mg/kg liposomal PLP resulted in a strong and lasting resolution of joint inflammation. 10 mg/kg free PLP only became slightly effective after repeated daily injections. Although joint inflammation recurred 1 week after treatment with liposomal PLP, knee joint sections prepared at this time indicated that the cartilage damage was still reduced. Localisation of gold labelled liposomes in the inflamed joints was seen in the proximity of blood vessels, in the cellular infiltrate, but mainly in the synovial lining. Unaffected joints did not take up liposomes. CONCLUSIONS: By using the property of long-circulating liposomes to target the synovial lining selectively in inflamed joints, the anti-inflammatory activity of GC can be greatly increased, showing also the beneficial effect of reduced cartilage destruction.
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Authors | J M Metselaar, W B van den Berg, A E M Holthuysen, M H M Wauben, G Storm, P L E M van Lent |
Journal | Annals of the rheumatic diseases
(Ann Rheum Dis)
Vol. 63
Issue 4
Pg. 348-53
(Apr 2004)
ISSN: 0003-4967 [Print] England |
PMID | 15020326
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Glucocorticoids
- Liposomes
- prednisolone phosphate
- Prednisolone
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Topics |
- Animals
- Anti-Inflammatory Agents
(administration & dosage)
- Arthritis, Experimental
(drug therapy, pathology)
- Glucocorticoids
(administration & dosage)
- Hindlimb
- Injections, Intravenous
- Joints
(drug effects, pathology)
- Liposomes
- Male
- Mice
- Mice, Inbred DBA
- Prednisolone
(administration & dosage, analogs & derivatives)
- Spleen
(pathology)
- Synovial Membrane
(drug effects, pathology)
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