Resistin messenger-RNA expression is increased by proinflammatory cytokines in vitro.

Abstract

Resistin is a recently discovered polypeptide that induces insulin resistance in rodents. While in rodents resistin is predominantly expressed in adipocytes, in humans peripheral blood mononuclear cells (PBMC) seem to a be a major source of resistin. In the present study, we show that in human PBMC resistin mRNA expression-determined by fluorescence-based real-time polymerase chain reaction-is strongly increased by the proinflammatory cytokines interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF-alpha), and also by lipopolysaccharides (LPS), respectively, while no effect was found by interferon-gamma (IFN-gamma) or leptin. Our results suggest that in humans resistin may be a link in the well-known association between inflammation and insulin resistance.

• Authors: S Kaser, A Kaser, A Sandhofer, C F Ebenbichler, H Tilg, J R Patsch
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 309 Issue 2 Pg. 286-90 (Sep 19 2003) ISSN: 0006-291X [Print] United States
• PMID: 12951047 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokines
• Cytokines
• Hormones, Ectopic
• Intercellular Signaling Peptides and Proteins
• RETN protein, human
• RETNLB protein, human
• RNA, Messenger
• Resistin

Topics

• Chemokines (pharmacology)
• Cytokines (pharmacology)
• Dose-Response Relationship, Drug
• Gene Expression Regulation (drug effects)
• Hormones, Ectopic (biosynthesis, genetics)
• Humans
• Inflammation (metabolism)
• Insulin Resistance
• Intercellular Signaling Peptides and Proteins
• Leukocytes, Mononuclear (drug effects, metabolism)
• RNA, Messenger (genetics, metabolism)
• Resistin