Abstract | OBJECTIVE: PATIENTS AND MEASUREMENTS: Thyroid tissues obtained at surgery from patients with various thyroid disorders (normal thyroid adjacent to adenoma, six cases; Graves' disease thyroid tissue, 25 cases; adenoma, eight cases; papillary carcinoma, 12 cases; Graves' disease peripheral blood lymphocytes, 11 cases) were used for RT assay, using a specific, improved assay system. RESULTS: Thyroid tissue extracts from patients with Graves' disease contained high RT activity which resembled that demonstrated in retroviruses. The RT existed in the thyroid tissue as a complex, with endogenous template RNA, and the activity was confirmed not to be due to other DNA polymerases. CONCLUSION: Retroviral RT distinguished from known cellular DNA polymerases is expressed in the thyroids of patients with Graves' disease. In a permissive genetice and immunological environment, retroviral DNA integrated into genomic DNA could precipitate the onset of Graves' disease.
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Authors | A Nagasaka, A Nakai, N Oda, M Kotake, K Iwase, S Yoshida |
Journal | Clinical endocrinology
(Clin Endocrinol (Oxf))
Vol. 53
Issue 2
Pg. 155-9
(Aug 2000)
ISSN: 0300-0664 [Print] England |
PMID | 10931095
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Biomarkers
- RNA-Directed DNA Polymerase
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Topics |
- Adenoma
(enzymology)
- Biomarkers
(analysis)
- Carcinoma, Papillary
(enzymology)
- Graves Disease
(enzymology)
- Humans
- Lymphocytes
(enzymology)
- RNA-Directed DNA Polymerase
(analysis)
- Retroviridae Infections
(enzymology)
- Statistics, Nonparametric
- Thyroid Gland
(enzymology)
- Thyroid Neoplasms
(enzymology)
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