Abstract | OBJECT: METHODS: Levels of NO and oxygen free radicals were determined in both reoxygenation in vitro and reperfusion in vivo models using an NO electrochemical probe and high-performance liquid chromatography with the 2,3- and 2,5-dihydroxybenzoic acid trapping method, before and after addition of the NO donor diethanolamine nitric oxide ( DEA/NO). Reoxygenation after anoxia produced a twofold increase in NO release by human fetal astrocytes and cerebral endothelial cells (p < 0.005). In both cell lines, there was also a two- to threefold increase in oxygen free radical production (p < 0.005). In human fetal astrocytes and cerebral endothelial cells given a single dose of DEA/NO, free radical production dropped fivefold compared with peak ischemic levels (p < 0.001). In a study in which a rat global cerebral ischemia model was used, NO production in a vehicle-treated group increased 48 +/- 16% above baseline levels after reperfusion. After intravenous DEA/NO infusion, NO reached 1.6 times the concentration of the postischemic peak in vehicle-treated animals. In vehicle-treated animals during reperfusion, free radical production increased 4.5-fold over basal levels (p < 0.01). After intravenous DEA/NO infusion, free radical production dropped nearly 10-fold compared with peak levels in vehicle-treated animals (p < 0.006). The infarct volume in the vehicle-treated animals was 111 +/- 16.9 mm3; after DEA/NO infusion it was 64.8 +/- 23.4 mm3 (p < 0.01). CONCLUSIONS:
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Authors | R B Mason, R M Pluta, S Walbridge, D A Wink, E H Oldfield, R J Boock |
Journal | Journal of neurosurgery
(J Neurosurg)
Vol. 93
Issue 1
Pg. 99-107
(Jul 2000)
ISSN: 0022-3085 [Print] United States |
PMID | 10883911
(Publication Type: Journal Article)
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Chemical References |
- Free Radicals
- Reactive Oxygen Species
- Nitric Oxide
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Topics |
- Adult
- Animals
- Astrocytes
(physiology)
- Brain
(blood supply)
- Cell Line
- Cerebral Infarction
(physiopathology)
- Chromatography, High Pressure Liquid
- Endothelium, Vascular
(physiopathology)
- Fetus
- Free Radicals
- Humans
- Hypoxia, Brain
(physiopathology)
- In Vitro Techniques
- Nitric Oxide
(physiology)
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(physiopathology)
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