Carcinoma-associated antigens MK-1 and CEA in urological cancers.

Abstract

BACKGROUND:

The MK-1 antigen, recognized by monoclonal antibody FU-MK-1, is widely associated with human carcinomas. However, the expression and distribution of MK-1 in urological cancers is not well known.

MATERIALS AND METHODS:

We examined the expression of MK-1 in 10 urological tumor cell lines using flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR) and in 15 cancer tissue specimens by immunohistochemical staining, and then compared it with that of carcinoembryonic antigen (CEA).

RESULTS:

When analyzed by flow cytometry, MK-1 was positive in 2 out of 3 bladder, 3 out of 3 prostate and one out of 4 renal tumor cell lines, whereas CEA was negative in all the 10 tumor cell lines. RT-PCR confirmed the presence of MK-1 mRNA in all the six MK-1-positive tumor cell lines. An immunohistochemical study demonstrated that MK-1 was positive in 2 out of 5 bladder, 2 out of 5 prostate and one out of 5 renal cancer tissues. Again, however, CEA was negative in all the 15 urological cancer tissues tested.

CONCLUSION:

These findings suggest that MK-1 seems to be a useful biological marker for malignant urological tumors, especially in cases of bladder and prostate cancer.

• Authors: Y Tomita, F Arakawa, Y Hirose, S Liao, P D Khare, M Kuroki, T Yamamoto, A Ariyoshi, M Kuroki
• Journal: Anticancer research (Anticancer Res) 2000 Mar-Apr Vol. 20 Issue 2A Pg. 793-7 ISSN: 0250-7005 [Print] Greece
• PMID: 10810356 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Biomarkers, Tumor
• Carcinoembryonic Antigen
• Cell Adhesion Molecules
• Epithelial Cell Adhesion Molecule

Topics

• Antibodies, Monoclonal
• Antigens, Neoplasm (analysis, genetics)
• Biomarkers, Tumor (analysis)
• Carcinoembryonic Antigen (analysis, genetics)
• Cell Adhesion Molecules (analysis, genetics)
• Epithelial Cell Adhesion Molecule
• Flow Cytometry
• Humans
• Immunohistochemistry
• Kidney (pathology)
• Kidney Neoplasms (genetics, pathology)
• Male
• Prostate (pathology)
• Prostatic Neoplasms (genetics, pathology)
• Reverse Transcriptase Polymerase Chain Reaction
• Stomach Neoplasms (pathology)
• Transcription, Genetic
• Tumor Cells, Cultured
• Urinary Bladder (pathology)
• Urinary Bladder Neoplasms (genetics, pathology)