Expression of nuclear factor-kappaB in Helicobacter pylori-infected gastric mucosa detected with southwestern histochemistry.

Abstract

BACKGROUND:

The transcription factor nuclear factor-kappaB (NF-kappaB) plays a pivotal role in inflammatory responses by upregulating mRNA expression of, for example, proinflammatory cytokines and chemokines. Although in vitro studies have shown that Helicobacter pylori can induce NF-kappaB activation in gastric cancer cell lines, there is little information on cellular localization of NF-kappaB in H. pylori-infected gastric mucosa.

METHODS:

H. pylori-infected and -negative patients with various endoscopic findings were examined. NF-kappaB expression was studied by means of Southwestern histochemistry, a new method of localizing transcription factors. Labeled double-stranded oligo-DNA with specific consensus sequence for the NF-kappaB binding site was reacted with frozen sections from gastric biopsy specimens. We compared mucosal interleukin-8 (IL-8) and IL-1beta levels as measured with enzyme-linked immunosorbent assay with the degree of NF-kappaB expression.

RESULTS:

NF-kappaB expression was often evident in nuclei of epithelial cells in H. pylori-infected gastric mucosa. The degree of NF-kappaB expression on the epithelium was significantly more severe in H. pylori-infected than in -negative mucosa. The degree of NF-kappaB expression also correlated with mucosal IL-8 levels but not with IL-8.

CONCLUSIONS:

H. pylori infection increases the expression of NF-kappaB in gastric mucosa, suggesting that NF-kappaB is involved in inflammatory responses to H. pylori.

• Authors: H Isomoto, M Miyazaki, Y Mizuta, F Takeshima, K Murase, K Inoue, K Yamasaki, I Murata, T Koji, S Kohno
• Journal: Scandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 35 Issue 3 Pg. 247-54 (Mar 2000) ISSN: 0036-5521 [Print] England
• PMID: 10766316 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Interleukin-1
• Interleukin-8
• NF-kappa B
• Oligonucleotide Probes

Topics

• Enzyme-Linked Immunosorbent Assay
• Female
• Gastric Mucosa (metabolism, microbiology)
• Gastritis (metabolism, microbiology)
• Helicobacter Infections (metabolism)
• Helicobacter pylori
• Humans
• Immunohistochemistry (methods)
• Interleukin-1 (biosynthesis)
• Interleukin-8 (biosynthesis)
• Male
• Middle Aged
• NF-kappa B (biosynthesis)
• Oligonucleotide Probes
• Peptic Ulcer (metabolism, microbiology)