Role of mast cell histamine in the formation of rat paw edema: a microdialysis study.

Abstract

We determined the endogenous histamine concentration in the subplantar space of rat hind paws using an in vivo microdialysis technique. A microdialysis probe was implanted into the rat hind paw and the histamine content in dialysates was measured by high performance liquid chromatography-fluorometry. In wild type (+/+) rats, the histamine output (basal level 25.7 +/- 0.9 pmol/ml) increased 115-, 199- and 426-fold rapidly after subplantar injection of compound 48/80 at doses of 0.5, 5 and 50 microg/paw, respectively. In genetically mast cell-deficient (Ws/Ws) rats, the basal level of histamine was one third of that obtained from +/+ rats, and was not increased by compound 48/80 injection. With this treatment, marked, dose dependent, but relatively gradual development of the paw edema was found in +/+ rats. However, no edema formation was observed in Ws/Ws rats. Histological observations showed neither mast cells nor edema to be present in the paw skin of Ws/Ws rats. These findings indicate the critical role of histamine as a trigger for the development of edema in vivo. In addition, Ws/Ws rats will provide important information as to the roles of mast cells in the inflammatory response.

• Authors: Y Guo, T Mochizuki, E Morii, Y Kitamura, K Maeyama
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 331 Issue 2-3 Pg. 237-43 (Jul 23 1997) ISSN: 0014-2999 [Print] Netherlands
• PMID: 9274985 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• p-Methoxy-N-methylphenethylamine
• Histamine

Topics

• Animals
• Cell Degranulation (drug effects)
• Chromatography, High Pressure Liquid
• Edema (metabolism, pathology)
• Fluorometry
• Foot (pathology)
• Histamine (metabolism)
• Histamine Release (drug effects)
• Male
• Mast Cells (drug effects, metabolism)
• Microdialysis
• Rats
• Rats, Inbred Strains
• p-Methoxy-N-methylphenethylamine (pharmacology)