[The effect of Staphylococcus aureus toxic shock exotoxin on whole blood cell chemiluminescence in vitro and in vivo].

Abstract

Stimulated and nonstimulated blood chemiluminescence were studied in presence of the toxic shock syndrome toxin I (TSST-I) in vivo and in vitro. In vivo experiments involved evaluation of rabbit whole blood chemiluminescence developed within various periods after the TSST-I intraperitoneal administration. Effect of various TSST-I concentrations on blood chemiluminescence was studied in vitro. TSST-I was shown to inhibit the nonstimulated chemiluminescence in blood within 45 min after administration of LD50 of both opsonized zymosan and calcium ionophore A 23187 in vivo. Similar effects the toxin exhibited at concentrations 20 microM and more in vitro. The maximum effect TSST-I demonstrated within the first 5 min of incubation and this effect was distinctly dose-dependent.

• Authors: A Iu Antipov, A P Shepelev, V M Poliakov
• Journal: Voprosy meditsinskoi khimii (Vopr Med Khim) 1995 Mar-Apr Vol. 41 Issue 2 Pg. 40-2 ISSN: 0042-8809 [Print] Russia (Federation)
• Vernacular Title: Vliianie ékzotoksina toksicheskogo shoka Staphylococcus aureus na khemiliuminestsentsiiu klekok tsel'noĭ krovi in vitro i in vivo.
• PMID: 7793095 (Publication Type: English Abstract, Journal Article)

Chemical References

• Bacterial Toxins
• Enterotoxins
• Superantigens
• enterotoxin F, Staphylococcal

Topics

• Animals
• Bacterial Toxins
• Enterotoxins (pharmacology)
• Female
• Lipid Peroxidation
• Luminescent Measurements
• Neutrophils (drug effects, metabolism)
• Rabbits
• Staphylococcus aureus (metabolism)
• Superantigens