Hematopoietic cell transplantation in fetal lambs with ceroid-lipofuscinosis.

Abstract

Hematopoietic cells from the liver of normal 45-48-day-old fetal lambs (Hb type AA) were transplanted intraperitoneally into 58-60-day-old recipient fetuses (Hb type BB). The recipient fetuses resulted from mating homozygous ceroid-lipofuscinosis affected males with heterozygous, phenotypically normal, females. The sex of the donor fetus was also recorded. At age 2 1/2 months the recipient lambs with ceroid-lipofuscinosis were diagnosed by histopathology of brain biopsies. Monitoring of blood and bone marrow cells showed that an average of 9% of blood cells in ceroid-lipofuscinosis affected recipients were of donor origin. No differences were evident in the clinical course of disease, brain weight, or histopathology of organs between transplanted and non-transplanted lambs with ceroid-lipofuscinosis. Under the conditions of this experiment, transplantation of fetal hematopoietic cells was not beneficial.

• Authors: V J Westlake, R D Jolly, B R Jones, D J Mellor, R Machon, E D Zanjani, W Krivit
• Journal: American journal of medical genetics (Am J Med Genet) Vol. 57 Issue 2 Pg. 365-8 (Jun 05 1995) ISSN: 0148-7299 [Print] United States
• PMID: 7668364 (Publication Type: Journal Article)

Topics

• Animals
• Atrophy
• Brain (pathology)
• Crosses, Genetic
• Female
• Fetal Tissue Transplantation
• Fetus
• Hematopoietic Stem Cell Transplantation
• Kidney (pathology)
• Liver (pathology)
• Male
• Neuronal Ceroid-Lipofuscinoses (pathology, therapy, veterinary)
• Pregnancy
• Retina (pathology)
• Sex Characteristics
• Sheep
• Sheep Diseases