Amniotic fluid phosphatidylglycerol: a predictor of fetal lung maturity using conventional one-dimensional thin-layer chromatography.

Abstract

We present a retrospective comparative analysis of 209 amniotic fluid samples and the neonatal outcome. The presence of phosphatidylglycerol in 159 transabdominal amniotic fluid samples and the associated lung status indicated a 98% prediction rate for absence of respiratory distress syndrome with 1.8% false-positive results corrected to 0%. Twenty-nine vaginal pool samples were 72% predictive of outcome with false-positive results corrected to 14%. These cases were mild, and if a phosphatidylglycerol level of greater than 3% is used to indicate lung maturity, the corrected false-positive rate is 0%. False-negative results are also corrected and discussed. We believe that predicting neonatal respiratory distress syndrome by phosphatidylglycerol determination, using readily available conventional one-dimensional thin-layer chromatography, is clinically reliable and can aid in determining the maturity of the lung regardless of site of fluid collection.

• Authors: J R Semmer, T R Traylor, E B Linton, P C Painter, M Meighan
• Journal: Southern medical journal (South Med J) Vol. 76 Issue 10 Pg. 1257-60 (Oct 1983) ISSN: 0038-4348 [Print] United States
• PMID: 6623138 (Publication Type: Journal Article)

Chemical References

• Phosphatidylglycerols

Topics

• Amniotic Fluid (analysis)
• Chromatography, Thin Layer
• Female
• Fetal Distress (diagnosis)
• Fetal Membranes, Premature Rupture (diagnosis)
• Fetal Organ Maturity
• Humans
• Lung (embryology)
• Phosphatidylglycerols (analysis)
• Pregnancy
• Prenatal Diagnosis