Abstract |
It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.
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Authors | I Takebe, Y Otsuki |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 64
Issue 3
Pg. 843-8
(Nov 1969)
ISSN: 0027-8424 [Print] United States |
PMID | 4905990
(Publication Type: Journal Article)
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Chemical References |
- Peptides
- Dactinomycin
- Thiouracil
- Chloramphenicol
- Cycloheximide
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Topics |
- Chloramphenicol
(pharmacology)
- Cycloheximide
(pharmacology)
- Cytopathogenic Effect, Viral
- Dactinomycin
(pharmacology)
- Fluorescent Antibody Technique
- Microscopy, Electron
- Microscopy, Fluorescence
- Peptides
(pharmacology)
- Plants, Toxic
- Protoplasts
(drug effects, microbiology)
- Thiouracil
(pharmacology)
- Tobacco
- Tobacco Mosaic Virus
(pathogenicity)
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