Preparation and labelling of red blood cells with [68Ga]Ga-oxine for PET/CT imaging of the human spleen.

Abstract

INTRODUCTION:

With the introduction of automated synthetization methods, the in-house production of several 68Ga-based tracers became feasible in hospital laboratories. We describe a possible standard operating procedure (SOP) for [68Ga]Ga-oxine-labeled heat-denaturated erythrocytes, which can be used for selective imaging in patients with splenic disorders.

METHODS:

Heat-denaturated erythrocytes were labeled with [68Ga]Ga-oxine, which was produced from 68Ga and 8-hydroxyquinoline on an automated synthesizer. The workflow was validated in a good manufacturing/good radiopharmaceutical practice (GMP/GRP) certified laboratory. A patient underwent [68Ga]Ga-oxine-erythrocyte PET/CT for differentiation of an intrapancreatic mass.

RESULTS:

[68Ga]Ga-oxine and [68Ga]Ga-oxine-labeled erythrocytes could be synthesized reproducibly and reliably. The products met GMP quality standards. The tracer showed high accumulation in the intrapancreatic mass, consistent with an accessory spleen.

CONCLUSIONS:

PET/CT imaging with [68Ga]Ga-oxine-labeled, heat-denaturated erythrocytes can be a backup method for the differentiation of functioning splenic tissue from tumors. An SOP for the production of the tracer in a clinical setting could be established.

• Authors: Robert Drescher, Sebastian Gröber, Martin Freesmeyer, Julia Greiser
• Journal: Nuclear medicine and biology (Nucl Med Biol) 2023 Mar-Apr Vol. 118-119 Pg. 108333 ISSN: 1872-9614 [Electronic] United States
• PMID: 36940567 (Publication Type: Letter)
• Copyright: Copyright © 2023 Elsevier Inc. All rights reserved.

Chemical References

• Oxyquinoline
• Gallium Radioisotopes

Topics

• Humans
• Positron Emission Tomography Computed Tomography (methods)
• Oxyquinoline
• Spleen (diagnostic imaging)
• Gallium Radioisotopes
• Erythrocytes