In this study heteroleptic A7B type novel Lu(iii) and Eu(iii)
lanthanide phthalocyanines (LnPc(Pox)[Pc'(AB3SH)]) with high extinction coefficients have been synthesized as candidate
photosensitizers with reaction yields higher than 33%. The
singlet oxygen quantum yields of LuPc(Pox)[Pc'(AB3SH)] and EuPc(Pox)[Pc'(AB3SH)], respectively, were measured 17% and 1.4% by the direct method in THF. The
singlet oxygen quantum yield of LuPc(Pox)[Pc'(AB3SH)] in THF is the highest among
lutetium(iii) bis-
phthalocyanine complexes to date. The photodynamic efficacy of the heteroleptic
lanthanide phthalocyanines was evaluated by measuring cell viabilities of A549 and BEAS-2B lung cells, selected to representing in vitro models for testing
cancer and normal cells against potential drugs. The cell viabilities demonstrated concentration dependent behavior and were varied by the type of phthalocyanines complexes. Irradiation of the cells for 30 minutes with LED array at 660 nm producing flux of 0.036 J cm-2 s-1 increased cell death for LuPcPox-OAc, LuPc(Pox)[Pc'(AB3SH)] and ZnPc. The IC50 concentrations of LuPc(Pox)[Pc'(AB3SH)] and ZnPc were determined to be below 10 nM for both cell lines, agreeing very well with the
singlet oxygen quantum yield measurements. These findings suggest that LuPc(Pox)[Pc'(AB3SH)] and particularly LuPcPox-OAc are promising
drug candidates enabling lowered dose and shorter irradiation time for
photodynamic therapy.