Abstract | BACKGROUND: There are limited effective prophylactic/early treatments for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Viral entry requires spike protein binding to the angiotensin-converting enzyme-2 receptor and cleavage by transmembrane serine protease 2 (TMPRSS2), a cell surface serine protease. Targeting of TMPRSS2 by either androgen blockade or direct inhibition is in clinical trials in early SARS-CoV-2 infection. METHODS: We used differentiated primary human airway epithelial cells at the air-liquid interface to test the impact of targeting TMPRSS2 on the prevention of SARS-CoV-2 infection. RESULTS: CONCLUSION: These experiments demonstrate a potential therapeutic role for topical camostat for pre- or post-exposure prophylaxis of SARS-CoV-2, which can now be evaluated in a clinical trial.
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Authors | Wenrui Guo, Linsey M Porter, Thomas Wm Crozier, Matthew Coates, Akhilesh Jha, Mikel McKie, James A Nathan, Paul J Lehner, Edward Jd Greenwood, Frank McCaughan |
Journal | Life science alliance
(Life Sci Alliance)
Vol. 5
Issue 4
(04 2022)
ISSN: 2575-1077 [Electronic] United States |
PMID | 35110354
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 Guo et al. |
Chemical References |
- Androgens
- Antiviral Agents
- Esters
- Guanidines
- Serine Proteinase Inhibitors
- camostat
- ACE2 protein, human
- Angiotensin-Converting Enzyme 2
- Serine Endopeptidases
- TMPRSS2 protein, human
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Topics |
- Administration, Topical
- Androgens
(metabolism)
- Angiotensin-Converting Enzyme 2
(genetics, metabolism)
- Antiviral Agents
(pharmacology)
- COVID-19
(prevention & control, virology)
- Cells, Cultured
- Epithelial Cells
- Esters
(pharmacology)
- Gene Expression
- Goblet Cells
(immunology, metabolism)
- Guanidines
(pharmacology)
- Host-Pathogen Interactions
(drug effects)
- Humans
- Respiratory Mucosa
(drug effects, metabolism, virology)
- SARS-CoV-2
(drug effects, physiology)
- Serine Endopeptidases
(genetics, metabolism)
- Serine Proteinase Inhibitors
(administration & dosage)
- Signal Transduction
- Virus Internalization
(drug effects)
- Virus Replication
(drug effects)
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