Hsa_circRNA_0001971 contributes to oral squamous cell carcinoma progression via miR-186-5p/Fibronectin type III domain containing 3B axis.

Abstract

BACKGROUND:

Circular RNAs (circRNAs) are closely associated with the progression of oral squamous cell carcinoma (OSCC). circRNA_0001971 has been proved to accelerate the OSCC development. Here, we aim to identify the new molecular mechanism of hsa_circRNA_0001971 (circRNA_0001971) in OSCC.

METHODS:

The levels of circRNA_0001971, miR-186-5p, and fibronectin type III domain containing 3B (FNDC3B) in tissues and cells were verified by qRT-PCR or Western blotting. The interaction between circRNA_0001971, miR-186-5p, and FNDC3B was identified by bioinformatics analysis, luciferase assay, and RIP assay. The effect of circRNA_0001971/miR-186-5p/FNDC3B axis on OSCC cell proliferation, migration, and invasion by cell functional experiments including CCK8, wound healing, and transwell assays.

RESULTS:

Our study displayed that circRNA_0001971 and FNDC3B were elevated in OSCC, whereas miR-186-5p was declined in OSCC. Silencing circRNA_0001971 attenuated the malignancy of OSCC cells by suppressing proliferation, migration, and invasion. In OSCC cells, circRNA_0001971 sponged miR-186-5p to enhance FNDC3B. Due to the interaction between circRNA_0001971, miR-186-5p, and FNDC3B, FNDC3B overexpression relieved the negative function of silencing circRNA_0001971 in OSCC cells.

CONCLUSION:

Overall, our study discovered that circRNA_0001971 was a tumor promoter in OSCC progression by targeting miR-186-5p/FNDC3B axis.

• Authors: Jiehua Zhang, Youjian Peng, Shengjun Jiang, Jun Li
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 36 Issue 3 Pg. e24245 (Mar 2022) ISSN: 1098-2825 [Electronic] United States
• PMID: 35060189 (Publication Type: Journal Article)
• Copyright: © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.

Chemical References

• FNDC3B protein, human
• Fibronectins
• MIRN186 microRNA, human
• MicroRNAs
• RNA, Circular

Topics

• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Fibronectins (genetics)
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs (genetics)
• Mouth Neoplasms (genetics, pathology)
• RNA, Circular (genetics)
• Squamous Cell Carcinoma of Head and Neck (genetics, pathology)