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Newly designed breakapart FISH probe helps to identify cases with true MECOM rearrangement in myeloid malignancies.

Abstract
A home-brew, tri-color MECOM breakapart FISH probe with a full MECOM coverage labeled with a separate dye is compared in parallel with a 2-color commercial MECOM breakapart probe in 17 cases of hematologic malignancies. Cases with a typical positive signal pattern (or "balanced" signal pattern) (n = 2) and a negative result (n = 3) using the commercial probe achieved the same results using the new probe (100% concordance), whereas 9 of 12 (75%) remaining cases with an atypical signal pattern (or "unbalanced" signal pattern) using the commercial probe showed a "balanced" signal pattern using the new probe. Three cases with undetermined MECOM rearrangement status by the commercial probe were further clarified with no MECOM rearrangement in 2 cases and presence of a subclone with simultaneous gain and rearrangement of MECOM in 1 case. More importantly, the new probe is capable of determining the presence, location and integrity of MECOM after rearrangement. In conclusion, atypical signal patterns obtained using a commercial FISH probe for MECOM can be solved through re-design and optimization of a new BAP probe, especially in those cases with a true MECOM rearrangement. The potential of the new probe for use in the clinical laboratory will be further investigated. (Word count: 196).
AuthorsMing Zhao, L Jeffrey Medeiros, Wei Wang, Guilin Tang, Hai Suk Jung, Steven M Sfamenos, Hong Fang, Gokce A Toruner, Shimin Hu, C Cameron Yin, Pei Lin, Jun Gu, Guang Peng, M James You, Joseph D Khoury, Sa A Wang, Zhenya Tang
JournalCancer genetics (Cancer Genet) Vol. 262-263 Pg. 23-29 (04 2022) ISSN: 2210-7762 [Print] United States
PMID34974290 (Publication Type: Journal Article)
CopyrightCopyright © 2021 Elsevier Inc. All rights reserved.
Chemical References
  • MDS1 and EVI1 Complex Locus Protein
  • MECOM protein, human
  • Transcription Factors
Topics
  • Chromosome Aberrations
  • Gene Rearrangement
  • Humans
  • MDS1 and EVI1 Complex Locus Protein
  • Myeloproliferative Disorders
  • Neoplasms
  • Transcription Factors

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