Abstract |
Dormant cancer cells that survive anticancer therapy can lead to cancer recurrence and disseminated metastases that prove fatal in most cases. Recently, specific dormant polyploid giant cancer cells (PGCC) have drawn our attention because of their association with the clinical risk of nasopharyngeal carcinoma (NPC) recurrence, as demonstrated by previous clinical data. In this study, we report the biological properties of PGCC, including mitochondrial alterations, and reveal that autophagy is a critical mechanism of PGCC induction. Moreover, pharmacologic or genetic inhibition of autophagy greatly impaired PGCC formation, significantly suppressing metastasis and improving survival in a mouse model. Mechanistically, chemotherapeutic drugs partly damaged mitochondria, which then produced low ATP levels and activated autophagy via the AMPK-mTOR pathway to promote PGCC formation. Analysis of the transcriptional and epigenetic landscape of PGCC revealed overexpression of RIPK1, and the scaffolding function of RIPK1 was required for AMPK-mTOR pathway-induced PGCC survival. High numbers of PGCCs correlated with shorter recurrence time and worse survival outcomes in patients with NPC. Collectively, these findings suggest a therapeutic approach of targeting dormant PGCCs in cancer. SIGNIFICANCE:
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Authors | Bo You, Tian Xia, Miao Gu, Zhenxin Zhang, Qicheng Zhang, Jianhong Shen, Yue Fan, Hui Yao, Si Pan, Yingna Lu, Tianyi Cheng, Zhiyuan Yang, Xin He, Hao Zhang, Muqi Shi, Dong Liu, Yiwen You |
Journal | Cancer research
(Cancer Res)
Vol. 82
Issue 5
Pg. 846-858
(Mar 01 2022)
ISSN: 1538-7445 [Electronic] United States |
PMID | 34965934
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | ©2021 The Authors; Published by the American Association for Cancer Research. |
Chemical References |
- MTOR protein, human
- TOR Serine-Threonine Kinases
- AMP-Activated Protein Kinases
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Topics |
- AMP-Activated Protein Kinases
(metabolism)
- Animals
- Autophagy
- Cell Line, Tumor
- Humans
- Mice
- Nasopharyngeal Carcinoma
(genetics)
- Nasopharyngeal Neoplasms
(metabolism)
- Neoplasm Recurrence, Local
- Polyploidy
- TOR Serine-Threonine Kinases
(metabolism)
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