LncRNA FOXP4-AS promotes the progression of non-small cell lung cancer by regulating the miR-3184-5p/EIF5A axis.

Abstract

Long non coding RNA FOXP4-AS1 exerted crucial functions in various human cancers, while its role in non-small cell lung cancer (NSCLC) remains unclear. A total of 30 pairs of NSCLC tissues and matched adjacent normal tissues were used to evaluate the expression of FOXP4-AS1 and miR-3184-5p. Cell proliferation was assessed by CCK-8 assay and colony formation assay. Cell apoptosis was measured by flow cytometry. Bioinformatic analysis and luciferase reporter assay were performed to determine the regulatory relationship among FOXP4-AS1, miR-3184-5p and EIF5A. The xenograft tumor model was constructed to confirm the function of FOXP4-AS1 in NSCLC progression. The results showed that FOXP4-AS1 was upregulated and miR-3184-5p was downregulated in NSCLC tissues and cell lines. Downregulation of FOXP4-AS1 significantly reduced cell proliferation and induced apoptosis of NSCLC cells in vitro. FOXP4-AS1 could regulated the expression of EIF5A by binding to miR-3184-5p. Rescue experiments showed that downregulation of miR-3184-5p or overexpression of EIF5A obviously attenuated the inhibitory effects of si-FOXP4-AS1 on cell proliferation, as well as the stimulating effects on cell apoptosis. Moreover, knockdown of FOXP4-AS1 could efficiently inhibited tumor development of NSCLC in vivo. Downregulation of FOXP4-AS1 attenuated the progression of NSCLC by regulating miR-3184-5p and EIF5A.

• Authors: Dingbiao Li, Zhenhua Li, Wang YanFei, Ying Wang, Jianlin Shi, Chang Liu, Laihao Qu, Shoujun Deng, Dalin Xiong
• Journal: Journal of tissue engineering and regenerative medicine (J Tissue Eng Regen Med) Vol. 16 Issue 4 Pg. 335-345 (04 2022) ISSN: 1932-7005 [Electronic] England
• PMID: 34921595 (Publication Type: Journal Article)
• Copyright: © 2021 The Authors. Journal of Tissue Engineering and Regenerative Medicine published by John Wiley & Sons Ltd.

Chemical References

• FOXP4 antisense RNA 1, human
• FOXP4 protein, human
• Forkhead Transcription Factors
• MIRN3184 microRNA, human
• MicroRNAs
• Peptide Initiation Factors
• RNA, Long Noncoding
• RNA-Binding Proteins

Topics

• Carcinoma, Non-Small-Cell Lung (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Forkhead Transcription Factors (genetics)
• Humans
• Lung Neoplasms (genetics, metabolism, pathology)
• MicroRNAs (genetics, metabolism)
• Peptide Initiation Factors
• RNA, Long Noncoding (genetics, metabolism)
• RNA-Binding Proteins
• Eukaryotic Translation Initiation Factor 5A