Abstract | BACKGROUND: PATIENTS &METHODS: A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank accession number NM_002520). RESULTS: Out of 50 patients with AML, 5 (10%) of them were NPM1 gene mutation positive, and 45 (90%) were negative. The mutation were a base substitution (C to A), (G to C), (G to T), transversion mutation in addition of frame shift mutation and all mutated cases were heterozygous and retained a wild type allele. CONCLUSION: Identification of NPM1 mutations in AML are important for prognostication, treatment decision and optimization of patient care.
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Authors | Galawezh Obaid Othman, Nawsherwan Sadiq Mohammad, Chiman Hameed Saeed |
Journal | African health sciences
(Afr Health Sci)
Vol. 21
Issue 2
Pg. 687-692
(Jun 2021)
ISSN: 1729-0503 [Electronic] Uganda |
PMID | 34795724
(Publication Type: Journal Article)
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Copyright | © 2021 Othman GO et al. |
Chemical References |
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Female
- Humans
- Iraq
- Leukemia, Myeloid, Acute
(genetics)
- Male
- Middle Aged
- Molecular Sequence Data
- Nucleophosmin
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Young Adult
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