LncSNHG3 promotes hepatocellular carcinoma epithelial mesenchymal transition progression through the miR-152-3p/JAK1 pathway.

Abstract

BACKGROUND:

The dysregulation of LncRNAs is related to the malignant progression of many cancers.

OBJECTIVE:

The study aimed to investigate the expression and the biological role of LncSNHG3 in hepatocellular carcinoma (HCC).

METHODS:

The TCGA data of the LncSNHG3 in HCC were analyzed. The expression in HCC cell lines was detected by qRT-PCR. Proliferation, migration, and invasion of HepG2 and Huh7 were examined by cell counting kit-8, colony formation, transwell assays, and wound healing assays. At the same time, the interactions among LncSNHG3, miR-152-3p, and JAK1 were confirmed by dual-luciferase reporter assay, RNA immunoprecipitation, subcellular distribution. Xenograft tumor-bearing mice models were used to measure the effect of LncSNHG3 on the growth of HCC in vivo. The apoptosis and epithelial mesenchymal transition (EMT)-associated proteins were checked by WB and IHC.

RESULTS:

LncSNHG3 was overexpressed in HCC tissues and cell lines. In addition, it is correlated with the tumor stage and survival time of HCC patients. Down-regulated LncSNHG3 could significantly suppress the EMT progression of HCC in vivo and in vitro. LncSNHG3 could promote the JAK1 expression by sponging miR-152-3p.

CONCLUSIONS:

LncSNHG3 acted as an oncogene and promoted the EMT procession in HCC by binding miR-152-3p and promoting JAK1 expression. Predictably, LncSNHG3 was used as a potential marker and will be used as a novel therapy target for HCC in the future.

• Authors: Hong Li, Yu Wu, Runmei Wang, Junmei Guo, Qin Yu, Lihe Zhang, Haiping Zhao, Hao Yang
• Journal: Genes & genomics (Genes Genomics) Vol. 44 Issue 1 Pg. 133-144 (Jan 2022) ISSN: 2092-9293 [Electronic] Korea (South)
• PMID: 34778942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Genetics Society of Korea.

Chemical References

• MIRN152 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• JAK1 protein, human
• Janus Kinase 1

Topics

• Animals
• Carcinoma, Hepatocellular (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Hep G2 Cells
• Humans
• Janus Kinase 1 (genetics, metabolism)
• Kaplan-Meier Estimate
• Liver Neoplasms (genetics, metabolism)
• Male
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics)
• Middle Aged
• RNA, Long Noncoding (genetics)
• Signal Transduction (genetics)
• Xenograft Model Antitumor Assays (methods)
• Mice