Potato Peels Mediated Synthesis of Cu(II)-nanoparticles from Tyrosinase Reacted with bis-(N-aminoethylethanolamine) (Tyr-Cu(II)-AEEA NPs) and Their Cytotoxicity against Michigan Cancer Foundation-7 Breast Cancer Cell Line.

Abstract

The synthesis of nanoparticles is most important in the context of cancer therapy, particularly copper nanoparticles, which are widely used. In this work, copper(II)-tyrosinase was isolated from potato peel powder. Copper nanoparticles (Tyr-Cu(II)-AEEA NPs) were synthesized via the reaction of tyrosinase with N-aminoethylethanolamine to produce Cu(II)-NPs and these were characterized by means of FT-IR, UV-Spectroscopy, XRD, SEM, TEM and a particle size analyzer. These Tyr-Cu(II)-AEEA NPs were tested as anticancer agents against MCF-7 breast cancer cells. Fluorescence microscopy and DNA fragmentation were also performed, which revealed the inhibiting potentials of Cu(II)-AEEA NPs and consequent cell death; Tyr-Cu(II)-AEEA NPs show potential cytotoxicity activity and this nano material could be contemplated as an anticancer medicament in future investigations.

• Authors: Akbar Idhayadhulla, Aseer Manilal, Anis Ahamed, Saud Alarifi, Gurusamy Raman
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 26 Issue 21 (Nov 03 2021) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 34771072 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Coordination Complexes
• Ethanolamines
• Copper
• Monophenol Monooxygenase

Topics

• Antineoplastic Agents (chemistry, metabolism, pharmacology)
• Cell Proliferation (drug effects)
• Coordination Complexes (chemistry, metabolism, pharmacology)
• Copper (chemistry, metabolism, pharmacology)
• Drug Screening Assays, Antitumor
• Ethanolamines (chemistry, metabolism, pharmacology)
• Female
• Humans
• MCF-7 Cells
• Metal Nanoparticles (chemistry)
• Microscopy, Fluorescence
• Monophenol Monooxygenase (metabolism)
• Solanum tuberosum (chemistry, metabolism)